Endocannabinoid mechanism for orofacial antinociception induced by electroacupuncture in acupoint St36 in rats

被引：8

作者：

Almeida, Renato Teixeira ^{[1
]}

Lima Romero, Thiago Roberto ^{[1
]}

Miranda E Castor Romero, Marina Gomes ^{[1
]}

de Souza, Giovane Galdino ^{[2
]}

Perez, Andrea de Castro ^{[1
]}

Gama Duarte, Igor Dimitri ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Dept Pharmacol, Inst Biol Sci, Belo Horizonte, MG, Brazil

[2] Univ Fed Alfenas, Dept Physiotherapy, Alfenas, MG, Brazil

来源：

PHARMACOLOGICAL REPORTS | 2016年 / 68卷 / 06期

关键词：

Electroacupuncture; Orofacial nociception; Endocannabinoid system; Antinociception; Acupoint St36; CANNABINOID RECEPTOR AGONISTS; NEUROPATHIC PAIN; PERIPHERAL ANTINOCICEPTION; ANANDAMIDE AMIDASE; BRAIN; CB1; MODEL; HYPERALGESIA; MODULATION; INHIBITION;

D O I：

10.1016/j.pharep.2016.07.004

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Introduction: This study was conducted with the aim of evaluating whether electroacupuncture (EA) at acupoint St36 could produce antinociception through the activation of an endocannabinoid mechanism. Methods: Male Wistar rats were divided into experimental groups. Heat was applied to the faces of rats, and the latency to withdraw the face was measured. Furthermore, the influence of electrical stimulation (100 HzP) of acupoint St36, at a 0.5 mA intensity, was investigated in the facial withdrawal threshold. Results: The EA produced antinociception, which lasted for 180 min. This effect was antagonized by the pre-injection of AM 251, a CB1 cannabinoid receptor antagonist, but not by AM 630, a CB2 cannabinoid receptor antagonist. Additionally, pretreatment with an endocannabinoid metabolizing enzyme inhibitor (MAFP) and an anandamide reuptake inhibitor (VDM11) prolonged and intensified the antinociceptive effect produced by EA. Conclusion: This study demonstrated for the first time that the CB1 cannabinoid receptor participates in the antinociceptive effect induced by EA. (C) 2016 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Sp. z o.o. All rights reserved.

引用

页码：1095 / 1101

页数：7

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