Fiber bundle endocytoscopy

被引:72
作者
Hughes, Michael [1 ,2 ]
Chang, Tou Pin [1 ,3 ]
Yang, Guang-Zhong [1 ,2 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Hamlyn Ctr Robot Surg, London SW2 2AZ, England
[2] Imperial Coll London UK, Dept Comp, London, England
[3] Imperial Coll London UK, Dept Surg & Canc, London, England
来源
BIOMEDICAL OPTICS EXPRESS | 2013年 / 4卷 / 12期
基金
英国工程与自然科学研究理事会;
关键词
CONFOCAL MICROSCOPY; 2-PHOTON MICROSCOPE; VIVO; ENDOMICROSCOPY; ESOPHAGUS; TECHNOLOGY;
D O I
10.1364/BOE.4.002781
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Endocytoscopy is an optical biopsy technique which uses a miniaturized camera to capture white light microscopy images through an endoscope. We have developed an alternative design that instead relays images to an external camera via a coherent fiber bundle. In this paper we characterize the device and demonstrate microscopy of porcine tissue ex vivo. One advantage of our approach is the ease with which other bundle-compatible imaging modalities can be deployed simultaneously. We show this by acquiring quasi-simultaneous endocytoscopy and fluorescence confocal endomicroscopy images through a single fiber bundle. This opens up possibilities for multi-modal endomicroscopy, combining white light and fluorescence imaging. (C) 2013 Optical Society of America
引用
收藏
页码:2781 / 2794
页数:14
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