Hydrogen-oxidizing hydrogenases 1 and 2 of Escherichia coli regulate the onset of hydrogen evolution and ATPase activity, respectively, during glucose fermentation at alkaline pH

被引：25

作者：

Poladyan, Anna ^{[1
]}

Trchounian, Karen ^{[2
,3
]}

Sawers, R. Gary ^{[3
]}

Trchounian, Armen ^{[1
]}

机构：

[1] Yerevan State Univ, Fac Biol, Dept Microbiol & Plants & Microbes Biotechnol, Yerevan 0025, Armenia

[2] Yerevan State Univ, Fac Biol, Dept Biophys, Yerevan 0025, Armenia

[3] Univ Halle Wittenberg, Inst Biol Microbiol, D-06108 Halle, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2013年 / 348卷 / 02期

关键词：

redox potential; hydrogenase; 1; 2; F0F1-ATPase; pH; Escherichia coli; H-2; EXPRESSION; GROWTH; ROLES;

D O I：

10.1111/1574-6968.12281

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Simultaneous measurement of redox potential (E-h) and determination of H-2 evolution kinetics using a pair of titanium silicate and platinum redox electrodes in fermenting cultures of Escherichia coli wild type and different mutants lacking hydrogenases 1 (Hyd-1) or 2 (Hyd-2) revealed that Hyd-1 controls the onset of H-2 evolution at slightly alkaline pH (pH 7.5) and under oxidizing E-h. In addition, Hyd-2 influences the N,N'-dicyclohexylcarbodiimide-inhibited ATPase activity in fermenting cells and thus regulates the proton F0F1-ATPase at the alkaline pH but under reducing E-h.

引用

页码：143 / 148

页数：6

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