Convergent Chemical Synthesis of Histone H2B Protein for the Site-Specific Ubiquitination at Lys34

被引：110
|
作者：
Siman, Peter [1 ]
Karthikeyan, Subramanian Vedhanarayanan [1 ]
Nikolov, Miroslav [2 ]
Fischle, Wolfgang [2 ]
Brik, Ashraf [1 ]
机构：
[1] Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel
[2] Max Planck Inst Biophys Chem, Lab Chromatin Biochem, D-37077 Gottingen, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 31期
基金：
以色列科学基金会;
关键词：
chromatin; H2B protein; peptide ligation; proteins; ubiquitination; LIGATION; STRATEGY; UBIQUITYLATION; METHYLATION; CROSSTALK; EFFICIENT; PEPTIDES; CYSTEINE;
D O I：
10.1002/anie.201303844
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
As You Like It: The convergent chemical synthesis of the histone H2B protein was achieved. This approach enabled the attachment of ubiquitin at Lys34 (see picture) and should make it possible to synthesize H2B with any other posttranslational modification in demand. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：8059 / 8063
页数：5
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