Immunogenicity of adenovirus and DNA vaccines co-expressing P39 and lumazine synthase proteins of Brucella abortus in BALB/c mice

被引:5
作者
Lin, Guo-Zhen [1 ,2 ]
Yang, Ju-Tian [2 ]
Wei, Suo-Cheng [2 ]
Chen, Shi-En [2 ]
Huo, Sheng-Dong [2 ]
Ma, Zhong-Ren [1 ]
机构
[1] Northwest Univ Nationalities, Key Bioengn & Technol Lab, Natl Nationality Commiss, Lanzhou 730030, Gansu, Peoples R China
[2] Northwest Univ Nationalities, Coll Life Sci & Engn, Lanzhou 730030, Gansu, Peoples R China
关键词
Adenovirus vaccine; BALB/c mice; Brucella abortus; DNAvaccine; Immunogenicity; Lumazine synthase; P39; PROTECTIVE EFFICACY; RIBOSOMAL-PROTEIN; INFECTION; OMP31; IMMUNIZATION; VACCINATION; STRATEGIES; IMMUNITY; L7/L12;
D O I
10.1007/s11250-018-1517-7
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Brucella poses a great threat to animal and human health. Vaccination is the most promising strategy in the effort to control Brucella abortus (B. abortus) infection, but the currently used live vaccines interfere with diagnostic tests and could potentially result in disease outbreak. Therefore, new subunit vaccines and combined immunization strategies are currently under investigation. In this study, immunogenicity and protection ability of a recombinant adenovirus and plasmid DNA vaccine co-expressing P39 and lumazine synthase proteins of B. abortus were evaluated based on the construction of the two molecular vaccines. Four immunization strategies (single adenovirus, single DNA, adenovirus/DNA, DNA/adenovirus) were investigated. The results showed that the immunization strategy of DNA priming followed by adenovirus boosting induced robust humoral and cellular immune responses, and it significantly reduced the numbers of B. abortus in a mouse model. These results suggest that it could be a potential antigen candidate for development of a new subunit vaccine against B. abortus infection.
引用
收藏
页码:957 / 963
页数:7
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