GAMETOPHYTIC FACTOR 1, Involved in Pre-mRNA Splicing, Is Essential for Megagametogenesis and Embryogenesis in Arabidopsis

被引：40

作者：

Liu, Man ^{[1
,2
]}

Yuan, Li ^{[1
,2
]}

Liu, Nai-You ^{[1
,2
]}

Shi, Dong-Qiao ^{[1
]}

Liu, Jie ^{[1
]}

Yang, Wei-Cai ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, Key Lab Mol & Dev Biol, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

来源：

JOURNAL OF INTEGRATIVE PLANT BIOLOGY | 2009年 / 51卷 / 03期

基金：

中国国家自然科学基金;

关键词：

embryogenesis; female gametophyte; pre-mRNA splicing; U5 snRNA complex; FEMALE GAMETOPHYTE DEVELOPMENT; SNRNP-SPECIFIC PROTEIN; POLLEN-TUBE GUIDANCE; SACCHAROMYCES-CEREVISIAE; U5; SNRNP; SEGREGATION DISTORTION; TRI-SNRNP; SPLICEOSOME; ENCODES; GENE;

D O I：

10.1111/j.1744-7909.2008.00783.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA biogenesis is essential and vital for accurate expression of genes. It is obvious that cells cannot continue normal metabolism when RNA splicing is interfered with. sgt13018 is such a mutant, with partial loss of function of GAMETOPHYTIC FACTOR 1 (GFA1); a gene likely involved in RNA biogenesis in Arabidopsis. The mutant is featured in the phenotype of diminished female gametophyte development at stage FG5 and is associated with the arrest of early embryo development in Arabidopsis. Bioinformatics data showed that homologs of gene GFA1 in yeast and human encode putative U5 snRNP-specific proteins required for pre-mRNA splicing. Furthermore, the result of yeast two-hybrid assay indicated that GFA1 physically interacted with AtBrr2 and AtPrp8, the putative U5 snRNP components, of Arabidopsis. This investigation suggests that GFA1 is involved in mRNA biogenesis through interaction with AtBrr2 and AtPrp8 and functions in megagametogenesis and embryogenesis in plant.

引用

页码：261 / 271

页数：11

共 51 条

[1] The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several US-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein [J].