Dynamic transcriptome profiling toward understanding the development of the human embryonic heart during different Carnegie stages

被引：4
作者：
Meng, Zhuo [1 ,2 ,3 ]
Wang, Jian [1 ]
Peng, Jiayu [1 ]
Zhou, Yue [1 ]
Zhou, Shuang [1 ]
Song, Wenting [1 ,2 ,3 ]
Chen, Sun [1 ]
Wang, Qingjie [1 ]
Bai, Kai [1 ]
Sun, Kun [1 ,2 ,3 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Pediat Cardiol, Shanghai 200000, Peoples R China
[2] Wenzhou Med Univ, Affiliated Hosp 2, Dept Pediat Cardiol, Wenzhou, Peoples R China
[3] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou, Peoples R China
来源：
FEBS LETTERS | 2020年 / 594卷 / 24期
基金：
中国国家自然科学基金; 上海市自然科学基金;
关键词：
carnegie stage; heart development; human embryonic heart; transcriptome; PLURIPOTENT STEM-CELLS; CARDIOMYOCYTES; MECHANISMS; SEPTATION; REVEALS; FIELD; EXPRESSION; MUTATIONS;
D O I：
10.1002/1873-3468.13930
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Transcriptional regulation participates in heart development. However, the transcriptomes of human embryonic hearts during Carnegie stage (CS)10-CS16 have not been elucidated. Here, we found marked changes in the morphology and transcriptome of the human embryonic heart from CS10 to CS11. At CS12-CS14, the embryonic heart undergoes hypoxia-to-aerobic transformation. At CS14-CS16, transcriptome functions were related to energy metabolism, regulation of cholesterol, and processes related to inorganic substances. Moreover, the transcriptomes of cardiac progenitor cells derived from human embryonic stem cells (hESCs) most overlapped with those of human embryonic hearts at CS10. Cardiomyocytes derived from hESCs considerably overlapped with embryonic hearts at CS14-CS16. Overall, these results provide a new perspective into the characteristics of human embryonic heart development.
引用
收藏
页码：4307 / 4319
页数：13
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