Enantioseparation of Substituted 1, 3-Diazaspiro [4.5]Decan-4-Ones: HPLC Comparative Study on Different Polysaccharide Type Chiral Stationary Phases

被引：7

作者：

Aboul-Enein, Hassan Y. ^{[1
]}

Aboul-Enein, Mohamed N. ^{[1
]}

El-Azzouny, Aida A. ^{[1
]}

Saleh, Ola A. ^{[1
]}

Hassan, Rasha M. ^{[1
]}

Amin, Kamilia M. ^{[2
]}

机构：

[1] Natl Res Ctr, Pharmaceut & Drug Ind Res Div, Pharmaceut & Med Chem Dept, Giza 12622, Egypt

[2] Cairo Univ, Fac Pharm, Dept Pharmaceut Chem, Cairo, Egypt

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2018年 / 56卷 / 02期

关键词：

LIQUID-CHROMATOGRAPHIC ENANTIOSEPARATION; FLUID CHROMATOGRAPHY; GAS-CHROMATOGRAPHY; SEPARATION; DERIVATIVES; ENANTIOMERS;

D O I：

10.1093/chromsci/bmx098

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Enantioseparation of substituted 1,3-diazaspiro[4.5]decan-4-ones (1-14) was achieved using different polysaccharide type chiral stationary phases (CSPs), namely, Chiralcel OJ, Chiralcel OD and Lux-Amylose-2 using different mobile phases which were either n-hexane/2-propanol or n-hexane/ethanol mixtures of various ratios (v/v) at flow rate 1 mL min(-1). UV detection was carried out at 254 nm and temperature of 20A degrees C. The retention behavior and selectivity of these CSPs were examined in isocratic normal phase high-performance liquid chromatography mode. The results revealed that the amylose CSP (Lux-Amylose-2) could separate almost all the compounds under investigation in contrast to cellulose CSPs (Chiracel OJ and Chiracel OD) which resolved fewer compounds.

引用

页码：160 / 165

页数：6

共 19 条

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