Multi-Organ Transcriptome Response of Lumpfish (Cyclopterus lumpus) to Aeromonas salmonicida Subspecies salmonicida Systemic Infection

被引：10

作者：

Chakraborty, Setu ^{[1
]}

Hossain, Ahmed ^{[1
]}

Cao, Trung ^{[1
]}

Gnanagobal, Hajarooba ^{[1
]}

Segovia, Cristopher ^{[1
]}

Hill, Stephen ^{[2
]}

Monk, Jennifer ^{[3
]}

Porter, Jillian ^{[3
]}

Boyce, Danny ^{[3
]}

Hall, Jennifer R. ^{[4
]}

Bindea, Gabriela ^{[5
,6
,7
]}

Kumar, Surendra ^{[1
,8
]}

Santander, Javier ^{[1
]}

机构：

[1] Mem Univ Newfoundland, Dept Ocean Sci, Marine Microbial Pathogenesis & Vaccinol Lab, St John, NL A1C 5S7, Canada

[2] Mem Univ Newfoundland, Dept Ocean Sci, Cold Ocean Deep Sea Res Facil, St John, NL A1C 5S7, Canada

[3] Mem Univ Newfoundland, Dept Ocean Sci, Dr Joe Brown Aquat Res Bldg, St John, NL A1C 5S7, Canada

[4] Mem Univ Newfoundland, Dept Ocean Sci, CREAIT Network, Aquat Res Cluster, St John, NL A1C 5S7, Canada

[5] Lab Integrat Canc Immunol, INSERM, F-75006 Paris, France

[6] Equipe Labellisee Ligue Canc, F-75013 Paris, France

[7] Univ Paris, Sorbonne Univ, Ctr Rech Cordeliers, F-75006 Paris, France

[8] Mem Univ Newfoundland, Ocean Frontier Inst, Dept Ocean Sci, St John, NL A1C 5S7, Canada

来源：

MICROORGANISMS | 2022年 / 10卷 / 11期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Aeromonas salmonicida infection; lumpfish immunity; multiorgan transcriptomics; gene discovery; RNA-SEQ DATA; SALMO-SALAR; COAGULATION; L; RECONSTRUCTION; STRATEGIES; IDENTIFICATION; VACCINATION; COMPLEMENT; METABOLISM;

D O I：

10.3390/microorganisms10112113

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lumpfish is utilized as a cleaner fish to biocontrol sealice infestations in Atlantic salmon farms. Aeromonas salmonicida, a Gram-negative facultative intracellular pathogen, is the causative agent of furunculosis in several fish species, including lumpfish. In this study, lumpfish were intraperitoneally injected with different doses of A. salmonicida to calculate the LD50. Samples of blood, head-kidney, spleen, and liver were collected at different time points to determine the infection kinetics. We determined that A. salmonicida LD50 is 10(2) CFU per dose. We found that the lumpfish head-kidney is the primary target organ of A. salmonicida. Triplicate biological samples were collected from head-kidney, spleen, and liver pre-infection and at 3- and 10-days post-infection for RNA-sequencing. The reference genome-guided transcriptome assembly resulted in 6246 differentially expressed genes. The de novo assembly resulted in 403,204 transcripts, which added 1307 novel genes not identified by the reference genome-guided transcriptome. Differential gene expression and gene ontology enrichment analyses suggested that A. salmonicida induces lethal infection in lumpfish by uncontrolled and detrimental blood coagulation, complement activation, inflammation, DNA damage, suppression of the adaptive immune system, and prevention of cytoskeleton formation.

引用

页数：32

共 133 条

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