Preparation and antioxidant activity of albumin binding Salen Schiff-base metal complexes

被引：13

作者：

Li Gang ^{[1
,2
]}

Zhang HuiFang ^{[1
]}

Wang RongMin ^{[1
]}

He YuFeng ^{[1
]}

Xiong YuBing ^{[1
]}

机构：

[1] Northwest Normal Univ, Key Lab Polymer Mat Gansu Prov, Key Lab Ecoenvironm Related Polymer Mat, Minist Educ,Inst Polymer, Lanzhou 730070, Peoples R China

[2] Lanzhou Inst Biol Prod, Lanzhou 730046, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2013年 / 58卷 / 24期

基金：

中国国家自然科学基金;

关键词：

bovine serum albumin; Schiff-base metal complexes; biopolymer metal complexes; hydroxyl radical scavenging activity; HUMAN SERUM-ALBUMIN; MIMICS; COPPER; WATER; SITE;

D O I：

10.1007/s11434-013-5787-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A kind of novel biopolymer antioxidant (BSA/HOSalenM, M=Co, Mn, Zn) is prepared with conjugation, which increases the antioxidant activity of the bovine serum albumin (BSA). The conjugations have been characterized by IR spectra, UV-Vis spectra, Fluorescence spectra, Circular dichroism (CD) spectra and Native-PAGE. The BSA is used as a biopolymer scaffold, and the insoluble Salen Schiff-base metal complexes HOSalenM make axial coordination with the amino acid residues of the BSA. The structure of the BSA is unchanged when the binding rate of HOSalenCo is less than 10. The HOSalenCo conjugations show an excellent hydroxyl radical (center dot OH) scavenging activity, and the activity (EC50) of BSA/HOSalenCo(10) (BSA:HOSalenCo=1:10) is improved by two orders of magnitude compared with the BSA, while the activity of the BSA/HOSalenMn is weak and the BSA/HOSalenZn shows no scavenging activity.

引用

页码：2956 / 2963

页数：8

共 33 条

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