D-β-Hydroxybutyrate inhibited the apoptosis of PC12 cells induced by H2O2 via inhibiting oxidative stress

被引：43

作者：

Cheng, Baohua ^{[1
]}

Lu, Hai ^{[1
]}

Bai, Bo ^{[1
]}

Chen, Jing ^{[1
]}

机构：

[1] Jining Med Univ, Dept Neurol, Jining 272067, Peoples R China

来源：

NEUROCHEMISTRY INTERNATIONAL | 2013年 / 62卷 / 05期

关键词：

D-beta-Hydroxybutyrate; Apoptosis; PC12; H2O2; Oxidative stress; PEROXIDE-INDUCED APOPTOSIS; NERVE GROWTH-FACTOR; HYDROGEN-PEROXIDE; PROTECTS; GLUTATHIONE; DAMAGE; CYTOTOXICITY; ALZHEIMERS; DEATH;

D O I：

10.1016/j.neuint.2012.09.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidative stress has an important role in neurodegenerative diseases and cerebral ischemic injury. It is reported that D-beta-hydroxybutyrate (D beta HB), the major component of ketone bodies, is neuroprotective in recent studies. Therefore, in the present work the neuroprotective effects of D beta HB on H2O2-induced apoptosis mediated by oxidative stress was investigated. PC12 cells were exposed to H2O2 with different concentrations of H2O2 for different times after D beta HB pretreatment. MTT assay, apoptotic rates, intracellular reactive oxygen species (ROS) level, GSH content, mitochondrial membrane potential (MMP) and caspase-3 activity were determined. The results showed that D beta HB inhibited the decrease of cell viability induced by H2O2 in PC12 cells. D beta HB decreased the apoptotic rates induced by H2O2. The changes of intracellular ROS, GSH, MMP and caspase-3 activity due to H2O2 exposure were partially reversed in PC12 cells. So D beta HB inhibited the apoptosis of PC12 cells induced by H2O2 via inhibiting oxidative stress. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：620 / 625

页数：6

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