Enzymatic hydrolysis of corn crop residues with high solid loadings: New insights into the impact of bioextrusion on biomass deconstruction using carbohydrate-binding modules

被引：26

作者：

Gatt, Etienne ^{[1
]}

Khatri, Vinay ^{[2
,4
]}

Bley, Julien ^{[3
,5
]}

Barnabe, Simon ^{[3
]}

Vandenbossche, Virginie ^{[1
]}

Beauregard, Marc ^{[3
,4
]}

机构：

[1] Univ Toulouse, LCA, INRA, Toulouse, France

[2] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada

[3] Univ Quebec Trois Rivieres, Ctr Rech Mat Lignocellulos, Trois Rivieres, PQ, Canada

[4] Univ Laval, PROTEO, Quebec City, PQ G1V 4G2, Canada

[5] Innofibre, 3351 Blvd Forges, Quebec City, PQ G9A 5E6, Canada

来源：

BIORESOURCE TECHNOLOGY | 2019年 / 282卷

关键词：

Lignocellulosic biomass; Bioextrusion; Carbohydrate-binding modules; Enzymatic hydrolysis; FTCM; FTCM-depletion assay; BIO-CATALYTIC ACTION; TWIN-SCREW EXTRUDER; CELLULOSE ACCESSIBILITY; ETHANOL FERMENTATION; ALKALI PRETREATMENT; DILUTE-ACID; LIGNOCELLULOSE; RECALCITRANCE; EXTRUSION; TRACKING;

D O I：

10.1016/j.biortech.2019.03.045

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Lignocellulosic biomass is a sustainable source of renewable substrate to produce low carbon footprint energy and materials. Biomass conversion is usually performed in two steps: a biomass pretreatment for improving cellulose accessibility followed by enzymatic hydrolysis of cellulose. In this study we investigated the efficiency of a bioextrusion pretreatment (extrusion in the presence of cellulase enzyme) for production of reducing sugars from corn crop agricultural residues. Our results demonstrate that bioextrusion increased the reducing sugar conversion yield by at least 94% at high solid/liquid ratio (14%-40%). Monitoring biomass surface with carbohydrate-binding modules (FTCM-depletion assay) revealed that well known negative impact of high solid/liquid ratio on conversion yield is not due to the lack of exposed cellulose which was abundant under such conditions. Bioextrusion was found to be less efficient on alkaline pretreated biomass but being a mild and solvent limiting pretreatment, it might help to minimize the waste stream.

引用

页码：398 / 406

页数：9

共 48 条

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