The Molecular Mechanism Underlying Mechanical Anisotropy of the Protein GB1

被引:27
作者
Li, Yongnan Devin [1 ]
Lamour, Guillaume [1 ,2 ]
Gsponer, Joerg [2 ]
Zheng, Peng [1 ]
Li, Hongbin [1 ]
机构
[1] Univ British Columbia, Dept Chem, Vancouver, BC, Canada
[2] Univ British Columbia, Ctr High Throughput Biol, Vancouver, BC, Canada
基金
加拿大自然科学与工程研究理事会; 加拿大创新基金会;
关键词
IMMUNOGLOBULIN-BINDING DOMAIN; FORCE SPECTROSCOPY REVEALS; SINGLE PROTEIN; DYNAMICS SIMULATIONS; ELASTOMERIC PROTEIN; METAL CHELATION; STABILITY; TITIN; POLYPROTEINS; ELASTICITY;
D O I
10.1016/j.bpj.2012.10.035
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Mechanical responses of elastic proteins are crucial for their biological function and nanotechnological use. Loading direction has been identified as one key determinant for the mechanical responses of proteins. However, it is not clear how a change in pulling direction changes the mechanical unfolding mechanism of the protein. Here, we combine protein engineering, single-molecule force spectroscopy, and steered molecular dynamics simulations to systematically investigate the mechanical response of a small globular protein GB1. Force versus extension profiles from both experiments and simulations reveal marked mechanical anisotropy of GB1. Using native contact analysis, we relate the mechanically robust shearing geometry with concurrent rupture of native contacts. This clearly contrasts the sequential rupture observed in simulations for the mechanically labile peeling geometry. Moreover, we identify multiple distinct mechanical unfolding pathways in two loading directions. Implications of such diverse unfolding mechanisms are discussed. Our results may also provide some insights for designing elastomeric proteins with tailored mechanical properties.
引用
收藏
页码:2361 / 2368
页数:8
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