Protective effects of an extract from Citrus bergamia against inflammatory injury in interferon-gamma and histamine exposed human keratinocytes

被引:40
作者
Graziano, Adriana C. E. [1 ]
Cardile, Venera [1 ]
Crasci, Lucia [2 ]
Caggia, Sivia [1 ]
Dugo, Paola [3 ]
Bonina, Francesco [2 ]
Panico, Annamaria
机构
[1] Univ Catania, Dept Biomed Sci, Physiol Sect, I-95125 Catania, Italy
[2] Univ Catania, Dept Drug Sci, I-95125 Catania, Italy
[3] Univ Messina, Dept Sci Alimenti & Ambiente, I-98166 Messina, Italy
关键词
Anti-inflammatory activity; Natural compound; Nitric oxide; ROS; ICAM-1; GAGs; INDUCED ICAM-1 EXPRESSION; NITRIC-OXIDE-SYNTHASE; WOUND REPAIR; NATURAL ANTIOXIDANTS; OXIDATIVE STRESS; IFN-GAMMA; FLAVONOIDS; MECHANISMS; RADIATION; RISSO;
D O I
10.1016/j.lfs.2012.04.043
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Aims: The present work evaluated the anti-inflammatory/antioxidant activity of a well characterized extract from Citrus bergamia Risso and Poiteau (CBE), containing neoeriocitrin, naringin, neohesperidin and other flavonoids, on human NCTC 2544 keratinocytes treated with interferon-gamma (IFN-gamma) and histamine (H). Main methods: High performance liquid chromatography (HPLC) coupled with diode array detectors was used to characterize and quantify phenolic compounds in CBE. Anti-inflammatory/antioxidant ability on keratinocytes was determined through evaluation of inter-cellular adhesion molecule-1 (ICAM-1) and inducible nitric oxide synthase (iNOS) expression by Western blot, production of nitric oxide (NO) with Griess reagent and concentration of reactive oxygen species (ROS) by fluorescent quantitative analysis with 2',7'-dichlorfluorescein-diacetate (DCFH-DA). Cell viability was assessed using 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Antioxidant activity was also measured by oxygen radical absorbance capacity (ORAC) assay. Glycosaminoglycans (GAGs) were quantified using 1.9-dimethyl methylene blue (DMB). Key findings: CBE exhibited high antioxidant activity confirmed by elevated ORAC values related to high capacity in oxygen radical scavenging. The assays on keratinocytes demonstrated that CBE does not inhibit cell proliferation and is shown to significantly reduce dose-dependently ICAM-1, iNOS, NO. ROS and GAG production in cells exposed to IFN-gamma and H. Significance: Our study demonstrates that the pools of compounds of an extract from C. bergainia efficiently block the proinflammatory actions induced by IFN-gamma and H on human keratinocytes. CBE may be used for topic employment in some inflammatory diseases of the skin and to represent an important opportunity for the essential oil processing industries. (c) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:968 / 974
页数:7
相关论文
共 45 条
[1]  
Albanesi C, 1999, J IMMUNOL, V162, P494
[2]   Reactive oxygen species and their detoxification in healing skin wounds [J].
auf dem Keller, Ulrich ;
Kuemin, Angelika ;
Braun, Susanne ;
Werner, Sabine .
JOURNAL OF INVESTIGATIVE DERMATOLOGY SYMPOSIUM PROCEEDINGS, 2006, 11 (01) :106-111
[3]   Constitutive nitric oxide synthase is present in normal human keratinocytes [J].
Baudouin, JE ;
Tachon, P .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1996, 106 (03) :428-431
[4]   Uses and properties of Citrus flavonoids [J].
Benavente-García, O ;
Castillo, J ;
Marin, FR ;
Ortuño, A ;
Del Río, JA .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (12) :4505-4515
[5]   Flavonoids differentially regulate IFNγ-induced ICAM-1 expression in human keratinocytes:: molecular mechanisms of action [J].
Bito, T ;
Roy, S ;
Sen, CK ;
Shirakawa, T ;
Gotoh, A ;
Ueda, M ;
Ichihashi, M ;
Packer, L .
FEBS LETTERS, 2002, 520 (1-3) :145-152
[6]   Natural Antioxidants: Sources, Compounds, Mechanisms of Action, and Potential Applications [J].
Brewer, M. S. .
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 2011, 10 (04) :221-247
[7]   OXYGEN-RADICAL ABSORBENCY CAPACITY ASSAY FOR ANTIOXIDANTS [J].
CAO, GH ;
ALESSIO, HM ;
CUTLER, RG .
FREE RADICAL BIOLOGY AND MEDICINE, 1993, 14 (03) :303-311
[8]  
Cardile V, 2005, ONCOL REP, V14, P981
[9]  
CERUTTI PA, 1991, CANCER CELL-MON REV, V3, P1
[10]  
Chahar Maheep K, 2011, Pharmacogn Rev, V5, P1, DOI 10.4103/0973-7847.79093