Single-cell Proteomics: Progress and Prospects

被引：241

作者：

Kelly, Ryan T. ^{[1
]}

机构：

[1] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA

来源：

MOLECULAR & CELLULAR PROTEOMICS | 2020年 / 19卷 / 11期

基金：

美国国家卫生研究院;

关键词：

ELECTROPHORESIS-MASS SPECTROMETRY; SUBAMBIENT PRESSURE IONIZATION; ELECTROSPRAY-IONIZATION; BOTTOM-UP; INTERFACE; MS; SENSITIVITY; EFFICIENCY; COVERAGE; PLATFORM;

D O I：

10.1074/mcp.R120.002234

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MS-based proteome profiling has become increasingly comprehensive and quantitative, yet a persistent short-coming has been the relatively large samples required to achieve an in-depth measurement. Such bulk samples, typically comprising thousands of cells or more, provide a population average and obscure important cellular heterogeneity. Single-cell proteomics capabilities have the potential to transform biomedical research and enable understanding of biological systems with a new level of granularity. Recent advances in sample processing, separations and MS instrumentation now make it possible to quantify >1000 proteins from individual mammalian cells, a level of coverage that required an input of thousands of cells just a few years ago. This review discusses important factors and parameters that should be optimized across the workflow for single-cell and other low-input measurements. It also highlights recent developments that have advanced the field and opportunities for further development.

引用

页码：1739 / 1748

页数：10

共 79 条

[1] Detection of 1 zmol injection of angiotensin using capillary zone electrophoresis coupled to a Q-Exactive HF mass spectrometer with an electrokinetically pumped sheath-flow electrospray interface [J].

Amenson-Lamar, Emily Ann ;

Sun, Liangliang ;

Zhang, Zhenbin ;

Bohn, Paul W. ;

Dovichi, Norman J. .

TALANTA, 2019, 204 :70-73

[2]

[Anonymous], 2008, PROTEOMICS SAMPLE PR

[3] Enhancing bottom-up and top-down proteomic measurements with ion mobility separations [J].

Baker, Erin Shammel ;

Burnum-Johnson, Kristin E. ;

Ibrahim, Yehia M. ;

Orton, Daniel J. ;

Monroe, Matthew E. ;

Kelly, Ryan T. ;

Moore, Ronald J. ;

Zhang, Xing ;

Theberge, Roger ;

Costello, Catherine E. ;

Smith, Richard D. .

PROTEOMICS, 2015, 15 (16) :2766-2776

[4] Proteomic Characterization of the Neural Ectoderm Fated Cell Clones in the Xenopus laevis Embryo by High-Resolution Mass Spectrometry [J].

Baxi, Aparna B. ;

Lombard-Banek, Camille ;

Moody, Sally A. ;

Nemes, Peter .

ACS CHEMICAL NEUROSCIENCE, 2018, 9 (08) :2064-2073

[5] A Compact Quadrupole-Orbitrap Mass Spectrometer with FAIMS Interface Improves Proteome Coverage in Short LC Gradients [J].

Bekker-Jensen, Dorte B. ;

Martinez-Val, Ana ;

Steigerwald, Sophia ;

Ruther, Patrick ;

Fort, Kyle L. ;

Arrey, Tabiwang N. ;

Harder, Alexander ;

Makarov, Alexander ;

Olsen, Jesper, V .

MOLECULAR & CELLULAR PROTEOMICS, 2020, 19 (04) :716-729

[6] Zeptomole-sensitivity electrospray ionization - Fourier transform ion cyclotron resonance mass spectrometry of proteins [J].

Belov, ME ;

Gorshkov, MV ;

Udseth, HR ;

Anderson, GA ;

Smith, RD .

ANALYTICAL CHEMISTRY, 2000, 72 (10) :2271-2279

[7] Understanding the tumor immune microenvironment (TIME) for effective therapy [J].

Binnewies, Mikhail ;

Roberts, Edward W. ;

Kersten, Kelly ;

Chan, Vincent ;

Fearon, Douglas F. ;

Merad, Miriam ;

Coussens, Lisa M. ;

Gabrilovich, Dmitry I. ;

Ostrand-Rosenberg, Suzanne ;

Hedrick, Catherine C. ;

Vonderheide, Robert H. ;

Pittet, Mikael J. ;

Jain, Rakesh K. ;

Zou, Weiping ;

Howcroft, T. Kevin ;

Woodhouse, Elisa C. ;

Weinberg, Robert A. ;

Krummel, Matthew F. .

NATURE MEDICINE, 2018, 24 (05) :541-550

[8] SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation [J].

Budnik, Bogdan ;

Levy, Ezra ;

Harmange, Guillaume ;

Slavov, Nikolai .

GENOME BIOLOGY, 2018, 19

[9] Simple and Integrated Spintip-Based Technology Applied for Deep Proteome Profiling [J].

Chen, Wendong ;

Wang, Shuai ;

Adhikari, Subash ;

Deng, Zuhui ;

Wang, Lingjue ;

Chen, Lan ;

Ke, Mi ;

Yang, Pengyuan ;

Tian, Ruijun .

ANALYTICAL CHEMISTRY, 2016, 88 (09) :4864-4871

[10] Defining the carrier proteome limit for single-cell proteomics [J].

Cheung, Tommy K. ;

Lee, Chien-Yun ;

Bayer, Florian P. ;

McCoy, Atticus ;

Kuster, Bernhard ;

Rose, Christopher M. .

NATURE METHODS, 2021, 18 (01) :76-+

← 1 2 3 4 5 6 7 8 →