RETRACTED: Comparative analysis of the Corynebacterium glutamicum transcriptome in response to changes in dissolved oxygen levels (Retracted Article)

被引：9
作者：
Liu, Xiuxia [1 ,2 ,3 ]
Yang, Sun [1 ,2 ,3 ]
Wang, Fen [1 ,2 ,3 ]
Dai, Xiaofeng [1 ,2 ,3 ]
Yang, Yankun [1 ,2 ,3 ]
Bai, Zhonghu [1 ,2 ,3 ]
机构：
[1] Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China
[2] Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China
[3] Jiangnan Univ, Sch Biotechnol, Key Lab Carbohydrate Chem & Biotechnol, Minist Educ, Wuxi 214122, Peoples R China
来源：
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2017年 / 44卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Corynebacterium glutamicum; Dissolved oxygen; Transcriptome; Metabolism; Bioprocess; METABOLIC FLUX; AMINO-ACIDS; RNA-SEQ; GENE; EXPRESSION; TOOL; VISUALIZATION; INACTIVATION; STRATEGY; SEQUENCE;
D O I：
10.1007/s10295-016-1854-3
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The dissolved oxygen (DO) level of a culture of Corynebacterium glutamicum (C. glutamicum) in a bioreactor has a significant impact on the cellular redox potential and the distribution of energy and metabolites. In this study, to gain a deeper understanding of the effects of DO on the metabolism of C. glutamicum, we sought to systematically explore the influence of different DO concentrations on genetic regulation and metabolism through transcriptomic analysis. The results revealed that after 20 h of fermentation, oxygen limitation enhanced the glucose metabolism, pyruvate metabolism and carbon overflow, and restricted NAD(+) availability. A high oxygen supply enhanced the TCA cycle and reduced glyoxylate metabolism. Several key genes involved in response of C. glutamicum to different oxygen concentrations were examined, which provided suggestions for target site modifications in developing optimized oxygen supply strategies. These data provided new insights into the relationship between oxygen supply and metabolism of C. glutamicum.
引用
收藏
页码：181 / 195
页数：15
相关论文
共 21 条
  • [21] Comparative transcriptome analysis reveals that chlorophyll metabolism contributes to leaf color changes in wucai (Brassica campestris L.) in response to cold
    Yuan, Lingyun
    Zhang, Liting
    Wu, Ying
    Zheng, Yushan
    Nie, Libing
    Zhang, Shengnan
    Lan, Tian
    Zhao, Yang
    Zhu, Shidong
    Hou, Jinfeng
    Chen, Guohu
    Tang, Xiaoyan
    Wang, Chenggang
    BMC PLANT BIOLOGY, 2021, 21 (01)
123