Decreased Expression of Na+/K+-ATPase, NHE3, NBC1, AQP1 and OAT in Gentamicin-induced Nephropathy

被引：10

作者：

Bae, Woo Kyun ^{[1
]}

Lee, JongUn ^{[2
]}

Park, Jeong Woo ^{[1
]}

Bae, Eun Hui ^{[1
]}

Ma, Seong Kwon ^{[1
]}

Kim, Suhn Hee ^{[3
]}

Kim, Soo Wan ^{[1
]}

机构：

[1] Chonnam Natl Univ, Sch Med, Dept Internal Med, Kwangju 501757, South Korea

[2] Chonnam Natl Univ, Sch Med, Dept Physiol, Kwangju 501757, South Korea

[3] Chonbuk Natl Univ, Sch Med, Dept Physiol, Jeonju 561756, South Korea

来源：

KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | 2008年 / 12卷 / 06期

关键词：

Gentamicin; Sodium transporters; Aquaporin-1; Organic anion transporters;

D O I：

10.4196/kjpp.2008.12.6.331

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The present study was aimed to determine whether there is an altered regulation of tubular transporters in gentamicin-induced nephropathy. Sprague-Dawley male rats (200 similar to 250 g) were subcutaneously injected with gentamicin (100 mg/kg per day) for 7 days, and the expression of tubular transporters was determined by immunoblotting and immunohistochemistry. The mRNA and protein expression of OAT was also determined. Gentamicin-treated rats exhibited significantly decreased creatinine clearance along with increased plasma creatinine levels. Accordingly, the fractional excretion of sodium increased. Urine volume was increased, while urine osmolality and free water reabsorption were decreased. Immunoblotting and immunohistochemistry revealed decreased expression of Na+/K+ -ATPase, NHE3, NBC1, and AQP1 in the kidney of gentamicin-treated rats. The expression of OAT1 and OAT3 was also decreased. Gentamicin-induced nephropathy may at least in part be causally related with a decreased expression of Na+/K+-ATPase, NHE3, NBC1, AQP1 and OAT.

引用

页码：331 / 336

页数：6

共 15 条

[1] Effects of α-lipoic acid on ischemia-reperfusion-induced renal dysfunction in rats [J].