mRNA level of alpha-2-macroglobulin as an aging biomarker of human fibroblasts in culture

被引：17
作者：
Ma, H [1 ]
Li, RZ [1 ]
Zhang, ZY [1 ]
Tong, TJ [1 ]
机构：
[1] Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, 38 Xueyuan Rd, Beijing 100083, Peoples R China
来源：
EXPERIMENTAL GERONTOLOGY | 2004年 / 39卷 / 03期
基金：
中国国家自然科学基金;
关键词：
cellular aging; biomarker; population doubling; alpha-2-macroglobulin; gene expression; cDNA array; Alzheimer's disease; fibroblasts; in vitro; RT-PCR;
D O I：
10.1016/j.exger.2003.11.012
中图分类号：
R592 [老年病学]; C [社会科学总论];
学科分类号：
03 ; 0303 ; 100203 ;
摘要：
Cellular senescence is a well-established model system for Studying the molecular basis of aging. To identify a reliable biomarker for cellular age and further study the gene expression of aging, we profiled the gene expression difference between aged and young cultured human embryonic lung fibroblasts by high-density complementary deoxyribonucleic acid (cDNA) arrays. Among the differentially expressed genes, alpha-2-macroglobulin (alpha(2)M) was selected for further study. Its gene expression level as a function of population doubling level (PDL) in cultured fibroblasts was determined by RT-PCR and northern hybridization. mRNA level of alpha(2)M showed a positive linear-correlation with cumulative PDL. Additional assays revealed that the levels of alpha(2)M increased in irreversible growth arrest induced by sublethal H2O2, but not in quiescent state of cultured fibroblasts induced by serum-deprivation, and remained stable in Hela cells. These results suggest that mRNA level of alpha(2)M can be used as a biomarker of aging in cultured fibroblasts. mRNA level of alpha(2)M showed significant difference between newborn and old human leucocytes, which Suggest that the mRNA level of alpha(2)M may be used as a biomarker of aging in vivo. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码：415 / 421
页数：7
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