Analysis of dipalmitoyl phosphatidylcholine in amniotic fluid by high-performance liquid chromatography

被引：2
作者：
Alvarez, JG
Slomovic, B
Ludmir, J
机构：
[1] Dept. of Obstetrics and Gynecology, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215
来源：
JOURNAL OF CHROMATOGRAPHY B | 1997年 / 690卷 / 1-2期
关键词：
dipalmitoyl phosphatidylcholine; lipids; FETAL LUNG MATURITY; RESPIRATORY-DISTRESS SYNDROME; FLUORESCENCE POLARIZATION ASSAY; PREDICTION; NUMBER;
D O I：
10.1016/S0378-4347(96)00415-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel method for the determination of dipalmitoyl phosphatidylcholine (DPPC) in amniotic fluid by high-performance liquid chromatography (HPLC) is described. Aliquots of 50 mu l of amniotic fluid were hydrolyzed with phospholipase C from Bacillus cereus and the resulting dipalmitoylglycerol analyzed by HPLC. Run-to-run and day-to-day precision for DPPC analysis were 4.2 and 6.1%, respectively, and analysis time was 10 min. Recoveries for DPPC ranged between 92 and 98%. In summarizing, this method provides a high precision and fast turnaround time means for the analysis of DPPC in amniotic fluid.
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收藏
页码：338 / 342
页数：5
相关论文
共 19 条
[1]   ANALYSIS OF DIPALMITOYL PHOSPHATIDYLCHOLINE IN AMNIOTIC-FLUID BY ENZYMATIC-HYDROLYSIS AND HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY REFLECTANCE SPECTRODENSITOMETRY [J].
ALVAREZ, JG ;
SLOMOVIC, B ;
LUDMIR, J .
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1995, 665 (01) :79-87
[2]   Prediction of respiratory distress syndrome by the novel dipalmitoyl phosphatidylcholine test [J].
Alvarez, JG ;
Richardson, DK ;
Ludmir, J .
OBSTETRICS AND GYNECOLOGY, 1996, 87 (03) :429-433
[3]   SEPARATION OF ACIDIC AND NEUTRAL LIPIDS BY AMINOPROPYL-BONDED SILICA-GEL COLUMN CHROMATOGRAPHY [J].
ALVAREZ, JG ;
TOUCHSTONE, JC .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 577 (01) :142-145
[4]  
ASHWOOD ER, 1990, OBSTET GYNECOL, V75, P289
[5]  
ASHWOOD ER, 1986, CLIN CHEM, V32, P260
[6]  
ASHWOOD ER, 1992, OBSTET GYNECOL, V80, P1048
[7]   LAMELLAR BODY NUMBER DENSITY AND THE PREDICTION OF RESPIRATORY-DISTRESS [J].
BOWIE, LJ ;
SHAMMO, J ;
DOHNAL, JC ;
FARRELL, E ;
VYE, MV .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1991, 95 (06) :781-786
[8]   ASSESSMENT OF RISK OF RESPIRATORY-DISTRESS SYNDROME BY A RAPID TEST FOR SURFACTANT IN AMNIOTIC-FLUID [J].
CLEMENTS, JA ;
CREASY, RK ;
HOBEL, CJ ;
TOOLEY, WH ;
TIERNEY, DF ;
THIBEAULT, DW ;
PLATZKER, AC ;
OH, W ;
MARGOLIS, AJ .
NEW ENGLAND JOURNAL OF MEDICINE, 1972, 286 (20) :1077-+
[9]  
DUBIN SB, 1989, CLIN CHEM, V35, P612
[10]  
FARRELL PM, 1975, AM REV RESPIR DIS, V111, P657
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