A putative new order of methanogenic Archaea inhabiting the human gut, as revealed by molecular analyses of the mcrA gene

被引：85
作者：
Mihajlovski, Agnes [1 ]
Alric, Monique [1 ]
Brugere, Jean-Francois [1 ]
机构：
[1] Univ Clermont1, UFR Pharm, CRNH, IFR Sante Auvergne,ERT CIDAM, F-63001 Clermont Ferrand, France
来源：
RESEARCH IN MICROBIOLOGY | 2008年 / 159卷 / 7-8期
关键词：
Methyl-coenzyme M reductase; Human intestinal microflora; Methanogens; Gut Microbiota;
D O I：
10.1016/j.resmic.2008.06.007
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The diversity of methanogenic Archaea from the gut of 6 humans was investigated by targeting mcrA, a molecular metabolic marker of methanogenesis. Three operational taxonomic units (OTUs) were recovered from about 400 clones analyzed, two of which were attributed to the expected Methanobacteriales Methanobrevibacter smithii (4 volunteers) and Methanosphaera stadtmanae (1 volunteer). The third OTU (1 Volunteer) corresponded to a distant phylotype that does not cluster with any of the five methanogenic orders. This result, also supported by 16S archaeal sequences retrieved from the same volunteer, strongly suggests there may be a sixth order and hence potential underestimation of the role of methanogens in gut physiology. (C) 2008 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：516 / 521
页数：6
相关论文
共 23 条
  • [1] BASIC LOCAL ALIGNMENT SEARCH TOOL
    ALTSCHUL, SF
    GISH, W
    MILLER, W
    MYERS, EW
    LIPMAN, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) : 403 - 410
  • [2] Bapteste Eric, 2005, Archaea, V1, P353, DOI 10.1155/2005/859728
  • [3] Chaban B, 2006, CAN J MICROBIOL, V52, P73, DOI [10.1139/WDS-147, 10.1139/w05-147]
  • [4] MOST PROBABLE NUMBER ENUMERATION OF H-2-UTILIZING ACETOGENIC BACTERIA FROM THE DIGESTIVE-TRACT OF ANIMALS AND MAN
    DORE, J
    MORVAN, B
    RIEULESME, F
    GODEREL, I
    GOUET, P
    POCHART, P
    [J]. FEMS MICROBIOLOGY LETTERS, 1995, 130 (01) : 7 - 12
  • [5] Diversity of the human intestinal microbial flora
    Eckburg, PB
    Bik, EM
    Bernstein, CN
    Purdom, E
    Dethlefsen, L
    Sargent, M
    Gill, SR
    Nelson, KE
    Relman, DA
    [J]. SCIENCE, 2005, 308 (5728) : 1635 - 1638
  • [6] The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis
    Fricke, WF
    Seedorf, H
    Henne, A
    Krüer, M
    Liesegang, H
    Hedderich, R
    Gottschalk, G
    Thauer, RK
    [J]. JOURNAL OF BACTERIOLOGY, 2006, 188 (02) : 642 - 658
  • [7] Axial differences in community structure of Crenarchaeota and Euryarchaeota in the highly compartmentalized gut of the soil-feeding termite Cubitermes orthognathus
    Friedrich, MW
    Schmitt-Wagner, D
    Lueders, T
    Brune, A
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (10) : 4880 - 4890
  • [8] Galtier N, 1996, COMPUT APPL BIOSCI, V12, P543
  • [9] PHYML Online - a web server for fast maximum likelihood-based phylogenetic inference
    Guindon, S
    Lethiec, F
    Duroux, P
    Gascuel, O
    [J]. NUCLEIC ACIDS RESEARCH, 2005, 33 : W557 - W559
  • [10] Identification of methyl coenzyme M reductase A (mcrA) genes associated with methane-oxidizing archaea
    Hallam, SJ
    Girguis, PR
    Preston, CM
    Richardson, PM
    DeLong, EF
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (09) : 5483 - 5491
123