Genetic tests for alleles of complementary-sex-determiner to support honeybee breeding programmes

被引:20
作者
Hyink, Otto [1 ,2 ]
Laas, Frans [3 ]
Dearden, Peter K. [1 ,2 ]
机构
[1] Univ Otago, Dept Biochem, Lab Evolut & Dev, Dunedin, New Zealand
[2] Univ Otago, Dept Biochem, Natl Res Ctr Growth & Dev, Dunedin, New Zealand
[3] Betta Bees Res Ltd, South Dunedin, New Zealand
关键词
genetic test; honeybee breeding; sex determination; high-resolution melting; HOMO-HETEROZYGOSITY; COLONY POPULATIONS; QUEENS;
D O I
10.1007/s13592-012-0181-6
中图分类号
Q96 [昆虫学];
学科分类号
摘要
The honeybee haplodiploid sex determination system depends on genetic variation at the complementary sex-determiner (csd) locus. In closed populations of honeybees, especially those undergoing selective breeding, the number of csd alleles can drop such that brood viability is affected. Here we present two polymerase chain reaction tests that allow the discrimination of csd alleles. Such tests should find utility in bee breeding programmes allowing the tracking and maintenance of csd alleles through successive generations.
引用
收藏
页码:306 / 313
页数:8
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