A High-Coverage Genome Sequence from an Archaic Denisovan Individual

被引：1240

作者：

Meyer, Matthias ^{[1
]}

Kircher, Martin ^{[1
]}

Gansauge, Marie-Theres ^{[1
]}

Li, Heng ^{[2
]}

Racimo, Fernando ^{[1
]}

Mallick, Swapan ^{[2
,3
]}

Schraiber, Joshua G. ^{[4
]}

Jay, Flora ^{[4
]}

Pruefer, Kay ^{[1
]}

de Filippo, Cesare ^{[1
]}

Sudmant, Peter H. ^{[6
]}

Alkan, Can ^{[5
,6
]}

Fu, Qiaomei ^{[1
,7
]}

Do, Ron ^{[2
]}

Rohland, Nadin ^{[2
,3
]}

Tandon, Arti ^{[2
,3
]}

Siebauer, Michael ^{[1
]}

Green, Richard E. ^{[8
]}

Bryc, Katarzyna ^{[3
]}

Briggs, Adrian W. ^{[3
]}

Stenzel, Udo ^{[1
]}

Dabney, Jesse ^{[1
]}

Shendure, Jay ^{[6
]}

Kitzman, Jacob ^{[6
]}

Hammer, Michael F. ^{[9
]}

Shunkov, Michael V. ^{[10
]}

Derevianko, Anatoli P. ^{[10
]}

Patterson, Nick ^{[2
]}

Andres, Aida M. ^{[1
]}

Eichler, Evan E. ^{[6
,11
]}

Slatkin, Montgomery ^{[4
]}

Reich, David ^{[2
,3
]}

Kelso, Janet ^{[1
]}

Paeaebo, Svante ^{[1
]}

机构：

[1] Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, D-04103 Leipzig, Germany

[2] Broad Inst Massachusetts Inst Technol & Harvard, Cambridge, MA 02142 USA

[3] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

[4] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA

[5] Bilkent Univ, Dept Comp Engn, TR-06800 Ankara, Turkey

[6] Univ Washington, Sch Med, Dept Genome Sci, Seattle, WA 98195 USA

[7] Chinese Acad Sci, Inst Vertebrate Paleontol & Paleoanthropol, CAS MPS Joint Lab Human Evolut, Beijing 100044, Peoples R China

[8] Univ Calif Santa Cruz, Jack Baskin Sch Engn, Santa Cruz, CA 95064 USA

[9] Univ Arizona, Arizona Res Labs, Div Biotechnol, Tucson, AZ 85721 USA

[10] Russian Acad Sci, Siberian Branch, Inst Archaeol & Ethnog, Palaeolith Dept, Novosibirsk 630090, Russia

[11] Univ Washington, Howard Hughes Med Inst, Seattle, WA 98195 USA

来源：

SCIENCE | 2012年 / 338卷 / 6104期

关键词：

NEANDERTHAL GENOME; PATTERNS; HISTORY; HOMININ; SIBERIA; ORIGIN; ASIA;

D O I：

10.1126/science.1224344

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We present a DNA library preparation method that has allowed us to reconstruct a high-coverage (30x) genome sequence of a Denisovan, an extinct relative of Neandertals. The quality of this genome allows a direct estimation of Denisovan heterozygosity indicating that genetic diversity in these archaic hominins was extremely low. It also allows tentative dating of the specimen on the basis of "missing evolution" in its genome, detailed measurements of Denisovan and Neandertal admixture into present-day human populations, and the generation of a near-complete catalog of genetic changes that swept to high frequency in modern humans since their divergence from Denisovans.

引用

页码：222 / 226

页数：5

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