Wnt/β-catenin signaling induces the aging of mesenchymal stem cells through promoting the ROS production

被引：131

作者：

Zhang, Da-yong ^{[1
]}

Pan, Yu ^{[1
]}

Zhang, Chong ^{[1
]}

Yan, Bing-xi ^{[1
]}

Yu, Shan-shan ^{[1
]}

Wu, Dong-ling ^{[1
]}

Shi, Meng-meng ^{[1
]}

Shi, Kai ^{[1
]}

Cai, Xin-xiao ^{[1
]}

Zhou, Shuang-shuang ^{[1
]}

Wang, Jun-bo ^{[1
]}

Pan, Jian-ping ^{[1
]}

Zhang, Li-huang ^{[1
]}

机构：

[1] Zhejiang Univ City Coll, Dept Basic Med, Sch Med & Life Sci, 51 Huzhou St, Hangzhou 310015, Zhejiang, Peoples R China

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2013年 / 374卷 / 1-2期

关键词：

Mesenchymal stem cells (MSCs); Aging; Wnt/beta-catenin signaling; ROS; OXIDATIVE DNA-DAMAGE; CELLULAR SENESCENCE; WNT; PATHWAY; DIFFERENTIATION; ACTIVATION; MECHANISMS; DISEASE; CANCER; P53;

D O I：

10.1007/s11010-012-1498-1

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recent studies have demonstrated that the Wnt/beta-catenin signaling plays an important role in stem cell aging. However, the mechanisms of cell senescence induced by Wnt/beta-catenin signaling are still poorly understood. Our preliminary study has indicated that activated Wnt/beta-catenin signaling can induce MSC aging. In this study, we reported that the Wnt/beta-catenin signaling was a potent activator of reactive oxygen species (ROS) generation in MSCs. After scavenging ROS with N-acetylcysteine, Wnt/beta-catenin signaling-induced MSC aging was significantly attenuated and the DNA damage and the expression of p16(INK4A), p53, and p21 were reduced in MSCs. These results indicated that the Wnt/beta-catenin signaling could induce MSC aging through promoting the intracellular production of ROS, and ROS may be the main mediators of MSC aging induced by excessive activation of Wnt/beta-catenin signaling.

引用

页码：13 / 20

页数：8

共 30 条

[1] Increased Wnt signaling during aging alters muscle stem cell fate and increases fibrosis [J].