A cross-sectional study on canine Leishmania (L.) infantum chagasi infection in Amazonian Brazil ratifies a higher prevalence of specific IgG-antibody response than delayed-type hypersensitivity in symptomatic and asymptomatic dogs

被引:14
作者
Silveira, Fernando T. [1 ,2 ]
Carneiro, Liliane A. [1 ]
Ramos, Patricia K. S. [1 ]
Chagas, Eugenia J. [3 ]
Lima, Luciana V. R. [1 ]
Campos, Marliane B. [1 ]
Laurenti, Marcia D. [4 ]
Gomes, Claudia M. C. [4 ]
Corbett, Carlos E. P. [4 ]
机构
[1] Minist Hlth, Evandro Chagas Inst, Dept Parasitol, BR-67030000 Ananindeua, Para, Brazil
[2] Fed Univ Para, Inst Trop Med, BR-66059 Belem, Para, Brazil
[3] Hlth Care Secretary Barcarena Municipal, Dept Publ Hlth, Belem, Para, Brazil
[4] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo, Brazil
基金
英国惠康基金;
关键词
VISCERAL LEISHMANIASIS; IMMUNE-RESPONSE; SKIN-TEST; CUTANEOUS LEISHMANIASIS; PARASITE DENSITY; LYMPH-NODES; STATE; SERODIAGNOSIS; TRANSMISSION; AMAZONENSIS;
D O I
10.1007/s00436-012-2989-4
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
This was a cross-sectional study which analyzed the prevalence and the clinical and immunological spectrum of canine Leishmania (L.) infantum chagasi infection in a cohort of 320 mongrel dogs living in an endemic area of American visceral leishmaniasis in the Amazonian Brazil by using, mainly, the indirect fluorescence antibody test (IFAT-IgG) and the delayed-type hypersensitivity (DTH), and the parasite research by the popliteal lymph node aspiration. The IFAT and DTH reactivity recognized three different immune response profiles: (1) IFAT((+))/DTH(-) (107 dogs), (2) IFAT((-))/DTH(+) (18 dogs), and (3) IFAT((+))/DTH(+) (13 dogs), providing an overall prevalence of infection of 43 % (138/320). Thus, the specific prevalence of IFAT ((+)) /DTH ((-)) 33.4 % (107/320) was higher than those of IFAT ((-)) /DTH ((+)) 5.6 % (18/320) and IFAT ((+)) /DTH ((+)) 4.0 % (13/320). Moreover, the frequency of these profiles among 138 infected dogs showed that the IFAT ((+)) /DTH ((-)) rate of 77.5 % (107/138) was also higher than those of 13.0 % (18/138) of IFAT ((-)) /DTH ((+)) and 9.5 % (13/138) of IFAT ((+)) /DTH ((+)) rates. The frequency of asymptomatic dogs (76 %-105) was higher than those of symptomatic (16.6 %-23) and oligosymptomatic ones (7.4 %-10). A total of 16 (11.6 %) L. (L.) i. chagasi isolates were obtained from infected dogs, all from the IFAT ((+)) /DTH ((-)) profile: 41 % (9/22) from symptomatic, 33.3 % (3/9) from oligosymptomatic, and 5.2 % (4/76) from asymptomatic dogs. These findings strongly suggested that despite the higher frequency of asymptomatic dogs (76 %-105), the majority (72.4 %-76) was characterized by the IFAT ((+)) /DTH ((-)) profile with a doubtful immunogenetic resistance against infection.
引用
收藏
页码:1513 / 1522
页数:10
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