Electrochemical properties and square-wave voltammetric determination of pravastatin

The electrochemical reduction and adsorptive voltammetric behaviour of pravastatin have been studied by means of cyclic and square-wave voltammetry at a hanging mercury-drop electrode in electrolytes of different pH. Within the entire pH range (2.0-9.0) in Britton Robinson buffer, pravastatin gave rise to a single voltammetric peak in the potential interval from -1.22 to -1.44 V, depending on pravastatin concentration. It was found that the reduction of pravastatin proceeds via a relatively stable intermediate, which is transformed to the final electroinactive product by a coupled chemical reaction or can be re-oxidized back to pravastatin. The rate of chemical transformation is controlled by the proton concentration. The electrode mechanism has the properties of a surface redox reaction. A sensitive analytical method for trace analysis of pravastatin based on the adsorptive stripping technique has been developed. The calibration plot was linear in the range 8 x 10(-8) -5 x 10(-7) mol L-1. Application of the square-wave voltammetric method to determination of pravastatin in a pharmaceutical dosage form, without sample pretreatment, resulted in acceptable deviation from the stated concentration.