Enzymatic polymerisation involving 2′-amino-LNA nucleotides

被引：8

作者：

Johannsen, Marie W. ^{[1
]}

Veedu, Rakesh N. ^{[1
,2
]}

Madsen, Andreas Stahl ^{[1
]}

Wengel, Jesper ^{[1
]}

机构：

[1] Univ So Denmark, Nucle Acid Ctr, Dept Phys Chem & Pharm, DK-5230 Odense M, Denmark

[2] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2012年 / 22卷 / 10期

基金：

新加坡国家研究基金会; 欧洲研究理事会;

关键词：

2 '-Amino-LNA; Locked nucleic acid; Modified nucleosides; Primer extension; Triphosphate; LOCKED NUCLEIC-ACID; THROMBIN BINDING APTAMER; KOD DNA-POLYMERASE; NUCLEOSIDE TRIPHOSPHATES; OLIGONUCLEOTIDES; MONOMERS; THERAPEUTICS; DUPLEXES; LIGANDS;

D O I：

10.1016/j.bmcl.2012.03.073

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The triphosphate of the thymine derivative of 2'-amino-LNA (2'-amino-LNA-TTP) was synthesised and found to be a good substrate for Phusion (R) HF DNA polymerase, allowing enzymatic synthesis of modified DNA encoded by an unmodified template. To complement this, 2'-amino-LNA-T phosphoramidites were incorporated into DNA oligonucleotides which were used as templates for enzymatic synthesis of unmodified DNA using either KOD, KOD XL or Phusion polymerases. 2'-Amino-LNA-T in the template and 2'-amino-LNA-TTP as a substrate both decreased reaction rate and yield compared to unmodified DNA, especially for sequences with multiple 2'-amino-LNA-T nucleotides. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.

引用

页码：3522 / 3526

页数：5

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