Enzymatic polymerisation involving 2′-amino-LNA nucleotides

被引：8
|
作者：
Johannsen, Marie W. [1 ]
Veedu, Rakesh N. [1 ,2 ]
Madsen, Andreas Stahl [1 ]
Wengel, Jesper [1 ]
机构：
[1] Univ So Denmark, Nucle Acid Ctr, Dept Phys Chem & Pharm, DK-5230 Odense M, Denmark
[2] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2012年 / 22卷 / 10期
基金：
新加坡国家研究基金会; 欧洲研究理事会;
关键词：
2 '-Amino-LNA; Locked nucleic acid; Modified nucleosides; Primer extension; Triphosphate; LOCKED NUCLEIC-ACID; THROMBIN BINDING APTAMER; KOD DNA-POLYMERASE; NUCLEOSIDE TRIPHOSPHATES; OLIGONUCLEOTIDES; MONOMERS; THERAPEUTICS; DUPLEXES; LIGANDS;
D O I：
10.1016/j.bmcl.2012.03.073
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
The triphosphate of the thymine derivative of 2'-amino-LNA (2'-amino-LNA-TTP) was synthesised and found to be a good substrate for Phusion (R) HF DNA polymerase, allowing enzymatic synthesis of modified DNA encoded by an unmodified template. To complement this, 2'-amino-LNA-T phosphoramidites were incorporated into DNA oligonucleotides which were used as templates for enzymatic synthesis of unmodified DNA using either KOD, KOD XL or Phusion polymerases. 2'-Amino-LNA-T in the template and 2'-amino-LNA-TTP as a substrate both decreased reaction rate and yield compared to unmodified DNA, especially for sequences with multiple 2'-amino-LNA-T nucleotides. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：3522 / 3526
页数：5
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