Cloning and characterization of two novel aldo-keto reductases (AKR1C12 and AKR1C13) from mouse stomach

被引：17

作者：

Ikeda, S

Okuda-Ashitaka, E

Masu, Y

Suzuki, T

Watanabe, K

Nakao, M

Shingu, K

Ito, S ^{[1
]}

机构：

[1] Kansai Med Univ, Dept Med Chem, Moriguchi, Osaka 5708506, Japan

[2] Kansai Med Univ, Dept Anesthesiol, Moriguchi, Osaka 5708506, Japan

[3] Takeda Chem Inst, Pharmacol Labs, Osaka 5328686, Japan

来源：

FEBS LETTERS | 1999年 / 459卷 / 03期

关键词：

cDNA cloning; hydroxysteroid dehydrogenase; phylogeny; stomach; immunohistochemistry;

D O I：

10.1016/S0014-5793(99)01243-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In contrast to hepatic hydrosteroid dehydrogenases (HSDs) of the aldo-keto reductase family (AKR1C), little is known about a stomach one. From a mouse stomach cDNA library, we isolated two clones encoding proteins of 323 amino acid residues. They exhibited 93.2% amino acid sequence identity and 64-68% with any known HSDs. Recombinant proteins expressed in Escherichia coli reduced 9,10-phenanthraquinone with NAD(P)H as cofactor. The mRNAs were exclusively expressed in stomach, liver and ileum, The present study demonstrates that these proteins are new members of the HSD subfamily and they are named AKR1C12 and AKR1C13, Immunohistochemical analysis suggests that they are involved in detoxification of xenobiotics in the stomach. (C) 1999 Federation of European Biochemical Societies.

引用

页码：433 / 437

页数：5

共 24 条

[1] PARASITISM BY THE SLOW BACTERIUM HELICOBACTER-PYLORI LEADS TO ALTERED GASTRIC HOMEOSTASIS AND NEOPLASIA [J].