A Dual-Response Fluorescent Probe Reveals the H2O2-Induced H2S Biogenesis through a Cystathionine -Synthase Pathway

被引：53

作者：

Yi, Long ^{[1
]}

Wei, Lv ^{[2
]}

Wang, Runyu ^{[2
]}

Zhang, Changyu ^{[1
]}

Zhang, Jie ^{[1
]}

Tan, Tianwei ^{[1
]}

Xi, Zhen ^{[2
]}

机构：

[1] BUCT, State Key Lab Organ Inorgan Composites, Beijing 100029, Peoples R China

[2] Nankai Univ, Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Natl Engn Res Ctr Pesticide Tianjin, Dept Chem Biol,State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2015年 / 21卷 / 43期

关键词：

fluorescent imaging; fluorescent probes; hydrogen peroxide; hydrogen sulfide; redox homeostasis; HYDROGEN-SULFIDE; OXIDATIVE STRESS; IMAGING H2S; L-CYSTEINE; REDOX; GLUTATHIONE; PEROXIDE; MECHANISMS; CHEMISTRY; BIOLOGY;

D O I：

10.1002/chem.201502832

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The two signaling molecules H2S and H2O2 play key roles in maintaining intracellular redox homeostasis. The biological relationship between H2O2 and H2S remains largely unknown in redox biology. In this study, we rationally designed and synthesized single- and dual-response fluorescent probes for detecting both H2O2 and H2S in living cells. The dual-response probe was shown to be capable of mono- and dual-detection of H2O2 and H2S selectively and sensitively. Detailed bioimaging studies based on the probes revealed that both exogenous and endogenous H2O2 could induce H2S biogenesis in living cells. By using gene-knockdown techniques with bioimaging, the H2S biogenesis was found to be majorly cystathionine -synthase (CBS)-dependent. Our finding shows the first direct evidence on the biological communication between H2O2 (ROS) and H2S (RSS) in vivo.

引用

页码：15167 / 15172

页数：6

共 56 条

[1] The Molecular Mechanism of the Catalase Reaction
Alfonso-Prieto, Mercedes
Biarnes, Xevi
Vidossich, Pietro
Rovira, Carme
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (33) : 11751 - 11761
[2] A highly selective and fast-response fluorescent probe for visualization of enzymatic H2S production in vitro and in living cells
不详
[J]. CHEMICAL COMMUNICATIONS, 2015, 51 (52) : 10463 - 10466
[3] [Anonymous], 2009, ANGEW CHEM, DOI DOI 10.1002/ANGE.200805693
[4] Redox outside the Box: Linking Extracellular Redox Remodeling with Intracellular Redox Metabolism
Banerjee, Ruma
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (07) : 4397 - 4402
[5] The cystine/cysteine cycle:: a redox cycle regulating susceptibility versus resistance to cell death
Banjac, A.
Perisic, T.
Sato, H.
Seiler, A.
Bannai, S.
Weiss, N.
Koelle, P.
Tschoep, K.
Issels, R. D.
Daniel, P. T.
Conrad, M.
Bornkamm, G. W.
[J]. ONCOGENE, 2008, 27 (11) : 1618 - 1628
[6] Cystathionine Beta-Synthase (CBS) Contributes to Advanced Ovarian Cancer Progression and Drug Resistance
Bhattacharyya, Sanjib
Saha, Sounik
Giri, Karuna
Lanza, Ian R.
Nair, K. Sreekumar
Jennings, Nicholas B.
Rodriguez-Aguayo, Cristian
Lopez-Berestein, Gabriel
Basal, Eati
Weaver, Amy L.
Visscher, Daniel W.
Cliby, William
Sood, Anil K.
Bhattacharya, Resham
Mukherjee, Priyabrata
[J]. PLOS ONE, 2013, 8 (11):
[7] Chemiluminescent probes for imaging H2S in living animals
Cao, J.
Lopez, R.
Thacker, J. M.
Moon, J. Y.
Jiang, C.
Morris, S. N. S.
Bauer, J. H.
Tao, P.
Mason, R. P.
Lippert, A. R.
[J]. CHEMICAL SCIENCE, 2015, 6 (03) : 1979 - 1985
[8] Chan J, 2012, NAT CHEM, V4, P973, DOI [10.1038/NCHEM.1500, 10.1038/nchem.1500]
[9] Fluorescent and colorimetric probes for detection of thiols
Chen, Xiaoqiang
Zhou, Ying
Peng, Xiaojun
Yoon, Juyoung
[J]. CHEMICAL SOCIETY REVIEWS, 2010, 39 (06) : 2120 - 2135
[10] Metallo-ROS in Alzheimer's disease:: Oxidation of neurotransmitters by CuII-β-amyloid and neuropathology of the disease
da Silva, Giordano F. Z.
Ming, Li-June
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (18) : 3337 - 3341

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