Differential responses of root growth, acid invertase activity and transcript level to copper stress in two contrasting populations of Elsholtzia haichowensis

被引:60
作者
Cai, Shenwen [1 ,2 ]
Xiong, Zhiting [1 ,2 ]
Li, Ling [1 ,2 ]
Li, Minjing [3 ]
Zhang, Luan [1 ,2 ]
Liu, Chen [1 ,2 ]
Xu, Zhongrui [1 ,2 ]
机构
[1] Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430072, Hubei, Peoples R China
[2] Wuhan Univ, Hubei Biomass Resource Chem & Environm Biotechnol, Wuhan 430072, Hubei, Peoples R China
[3] China Univ Geosci, Sch Environm Studies, Wuhan 430074, Hubei, Peoples R China
关键词
Elsholtzia haichowensis; Root/shoot allometry; Root architecture; Acid invertase; Copper; RUMEX-DENTATUS L; THLASPI-CAERULESCENS; SYSTEM ARCHITECTURE; PLANT DEVELOPMENT; METAL TOLERANCE; CU STRESS; ACCUMULATION; MECHANISMS; ALLOCATION; ALLOMETRY;
D O I
10.1007/s10646-013-1153-y
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
The present study aimed to test a hypothesis that acid invertases in root of metallophytes might play important roles in root growth under heavy metal stress. Plants of two contrasting populations, one from an ancient Cu mine (MP) and the other from a non-contaminated site (NMP), of metallophyte Elsholtzia haichowensis were treated with Cu in controlled experiments. The results showed that MP was Cu tolerant under 10 mu M Cu2+ treatment. Cu treatment resulted in a higher root/shoot biomass ratio in MP compared to NMP. Scaling exponent in root/shoot allometric function in MP was lower than NMP. More complicated root architecture was observed in MP under Cu stress. Four full-length cDNAs (EhNcwINV, EhCcwINV, EhNvINV and EhCvINV) encoding cell wall and vacuolar invertases were cloned. Both of the transcript level and activity of the acid invertase in MP elevated under Cu treatment. There were positive correlations between root acid invertase transcript level, activity and root/shoot biomass ratio. The results indicated important roles of acid invertase in governing root growth under Cu stress. It also suggested that there was a possible interrelation between acid invertases and Cu tolerance mechanisms in MP of E. haichowensis.
引用
收藏
页码:76 / 91
页数:16
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