In vitro Antigenotoxicity of Ulva rigida C. Agardh (Chlorophyceae) Extract against Induction of Chromosome Aberration, Sister Chromatid Exchange and Micronuclei by Mutagenic Agent MMC

被引：20

作者：

Celikler, Serap ^{[1
]}

Yildiz, Gamze ^{[1
]}

Vatan, Ozgur ^{[1
]}

Bilaloglu, RAtimi ^{[1
]}

机构：

[1] Uludag Univ, Fac Sci & Arts, Dept Biol, TR-16059 Bursa, Turkey

来源：

BIOMEDICAL AND ENVIRONMENTAL SCIENCES | 2008年 / 21卷 / 06期

关键词：

Ulva rigida; Anticlastogenicity; Antigenotoxicity; Chromosomal aberration; Sister chromatid exchange; Micronuclei; Mitomycin-C;

D O I：

10.1016/S0895-3988(09)60008-8

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Objective To determine the in vitro possible clastogenic and cytotoxic activities of Ova rigida crude extracts (URE), and identify their antigenotoxic and protective effects on chemotherapeutic agent mitomycine-C (MMC). Methods Anti-clastogenic and anti-genotoxic activities of Ulva rigida crude extracts (URE) were studied using chromosome aberration (CA), sister chromatid exchange (SCE), and micronuclei (MN) tests in human lymphocytes cultured in vitro. Results The chromosome aberration, sister chromatid exchange or micronuclei tests showed that URE at concentrations of 10, 20, and 40 mu g/mL had no clastogenic activity in human lymphocyte cell Culture. Three doses of URE significantly decreased the number of chromosomal aberrations and the frequencies of SCE and MN when compared with the culture treated with MMC (P<0.0001). Conclusion Although URE itself is not a clastogenic or cytotoxic substance, it possesses strong antigenotoxic, anti-clastogenic, and protective effects on MMC in vitro.

引用

页码：492 / 498

页数：7

共 40 条

[1]

AARONSON S, 2000, IMPORTANT VEGETA 2 S, P231

[2]

[Anonymous], BIOTECHNOLOGY

[3]

[Anonymous], 1988, OCEANOGR MAR BIOL

[4]

[Anonymous], MARINE ALGAE PHARM S

[5] Screening of cultivated seaweeds for antibacterial activity against fish pathogenic bacteria [J].

Bansemir, A ;

Blume, M ;

Schröder, S ;

Lindequist, U .

AQUACULTURE, 2006, 252 (01) :79-84

[6] Seaweed, vitamin K, and warfarin [J].

Bartle, WR ;

Madorin, P ;

Ferland, G .

AMERICAN JOURNAL OF HEALTH-SYSTEM PHARMACY, 2001, 58 (23) :2300-2300

[7]

BENN PA, 1992, HUMAN CYTOGENETICS P, V1, P57

[8]

Bonassi S, 2001, ENVIRON MOL MUTAGEN, V37, P31

[9] DIETARY CAROTENES, VITAMIN-C, AND VITAMIN-E AS PROTECTIVE ANTIOXIDANTS IN HUMAN CANCERS [J].

BYERS, T ;

PERRY, G .

ANNUAL REVIEW OF NUTRITION, 1992, 12 :139-159

[10] CONSIDERATIONS FOR POPULATION MONITORING USING CYTOGENETIC TECHNIQUES [J].

CARRANO, AV ;

NATARAJAN, AT .

MUTATION RESEARCH, 1988, 204 (03) :379-406

← 1 2 3 4 →