A synthetic approach to GFP chromophore analogs from 3-azidocinnamates. Role of methyl rotors in chromophore photophysics

被引：32

作者：

Baranov, Mikhail S. ^{[1
]}

Solntsev, Kyril M. ^{[2
]}

Lukyanov, Konstantin A. ^{[1
]}

Yampolsky, Ilia V. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Bioorgan Chem, Moscow 117997, Russia

[2] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 51期

基金：

美国国家科学基金会;

关键词：

PROTEIN CHROMOPHORE; STATE DYNAMICS; FLUORESCENCE;

D O I：

10.1039/c3cc41948g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have suggested a novel combinatorial approach for synthesis of otherwise inaccessible GFP chromophore analogs, and studied the influence of aliphatic substituents on their pH-dependent spectral properties. We found that the demethylation at C or N positions of the imidazolone ring leads to a decrease in the excited state lifetime.

引用

页码：5778 / 5780

页数：3

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