Fluorescent Detection of Bovine Serum Albumin Using Surface Imprinted Films Formed on PDMS Microfluidic Channels

被引：5

作者：

Jeon, Sangheon ^{[1
]}

Kwon, Young Woo ^{[2
]}

Park, Jin Young ^{[3
]}

Hong, Suck Won ^{[1
]}

机构：

[1] Pusan Natl Univ, Coll Nanosci & Nanotechnol, Dept Opt & Mechatron Engn, Dept Cognomechatron Engn, Busandaehak Ro 63 Beon Gil 2, Busan 46241, South Korea

[2] Pusan Natl Univ, Dept Dept Nanofus Technol, Busandaehak Ro 63 Beon Gil 2, Busan 46241, South Korea

[3] Kyungpook Natl Univ, Dept Polymer Sci & Engn, 80 Daehak Ro, Daegu 41566, South Korea

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2019年 / 19卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

Molecularly Imprinted Polymer; Bovine Serum Albumin; Microfluidics; Fluorescence; POLYMER-FILMS; SYSTEM;

D O I：

10.1166/jnn.2019.16707

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this article, we describe a facile method to fabricate MIP patterns in specifically designed microfluidic channels. With this design, homogenous and stable MIP films were spatially immobilized inside the patterned PDMS channels. In this system, the fluorescent response was identified by detection of the fluorescence-labeled bovine serum albumin (BSA) template. In comparison, non-imprinted polymer (NIP) was also prepared. From the results of fluorescent response, significant binding behaviors of BSA molecules into the cavities of MIP patterns was observed due to the increased residence time in each ancillary hexagonal channel caused by the turbulent flow. However, the NIP patterns did not show the fluorescent response. Thus, the use of this system provides effective MIP-based microfluidic channels for the application of biosensors.

引用

页码：4736 / 4739

页数：4

共 12 条

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