The determination ofPlutella xylostella(L.)GSTs(PxGSTs) involved in the detoxification metabolism of Tolfenpyrad

被引：19

作者：

Li, Yifan ^{[1
]}

Sun, Hong ^{[1
]}

Tian, Zhen ^{[2
]}

Su, Xinxin ^{[1
]}

Li, Yue ^{[1
]}

Ye, Xuan ^{[1
]}

Zhou, Yifei ^{[1
]}

Zheng, Shengli ^{[3
]}

Liu, Jiyuan ^{[1
]}

Zhang, Yalin ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Plant Protect, Key Lab Plant Protect Resources & Pest Management, Minist Educ, Yangling 712100, Shaanxi, Peoples R China

[2] Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou, Jiangsu, Peoples R China

[3] Northwest A&F Univ, Coll Chem & Pharm, Yangling, Shaanxi, Peoples R China

来源：

PEST MANAGEMENT SCIENCE | 2020年 / 76卷 / 12期

基金：

中国国家自然科学基金;

关键词：

glutathione S-transferases (GSTs); Tolfenpyrad (TFP); molecular docking; site-directed mutagenesis; Plutella xylostella; GLUTATHIONE-S-TRANSFERASE; PLUTELLA-XYLOSTELLA L; DIAMONDBACK MOTH; INSECTICIDAL ACTIVITY; GENETIC ALGORITHM; RESISTANCE; IDENTIFICATION; LEPIDOPTERA; LARVAE; RECOGNITION;

D O I：

10.1002/ps.5958

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

BACKGROUND Insect glutathione S-transferases (GSTs) play a crucial role in insecticide detoxification. However, there remains a distinct lack of information regarding the role of GSTs in the detoxification of Tolfenpyrad (TFP) in insects. RESULTS Real-time quantitative PCR showed significant upregulation of PxGSTs after exposure to TFP for 6 h. Anin vitroinhibition assay showed that TFP could inhibit PxGST delta, PxGST epsilon and PxGST sigma, and the most pronounced inhibitory effect was on PxGST sigma. Metabolism assays displayed that PxGST sigma was superior to other test PxGSTs in metabolizing TFP. The molecular docking of TFP and PxGST sigma revealed that the H-bond provided by the sidechains of Tyr107 and Tyr162 were key to the detoxification of TFP by PxGST sigma. Further tests using mutant PxGST sigma proteins at the sites of Tyr107 (PxGST sigma Y107A) and Tyr162 (PxGST sigma Y162A) corroborated that the individual replacement of Tyr107 and Tyr162 could greatly weaken the binding and metabolic abilities to TFP. CONCLUSION Metabolic interactions between thePlutella xylostella(L.) GSTs (PxGSTs) and TFP were deciphered. This study illustrates the molecular metabolism mechanism of PxGST sigma towards TFP and provides theoretical underpinnings for the design and optimization of novel TFP-like insecticides.

引用

页码：4036 / 4045

页数：10

共 72 条

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