Crucial Roles of MicA and RybB as Vital Factors for σE-Dependent Cell Lysis in Escherichia coli Long-Term Stationary Phase

被引：12

作者：

Nagamitsu, Hiroshi ^{[1
]}

Murata, Masayuki ^{[1
]}

Kosaka, Tomoyuki ^{[2
]}

Kawaguchi, Junpei ^{[1
]}

Mori, Hirotada ^{[3
,4
]}

Yamada, Mamoru ^{[1
,2
]}

机构：

[1] Yamaguchi Univ, Grad Sch Med, Appl Mol Biosci, Ube, Yamaguchi 755, Japan

[2] Yamaguchi Univ, Dept Biol Chem, Fac Agr, Yamaguchi 7538515, Japan

[3] Nara Inst Technol, R&D Ctr Informat, Nara, Japan

[4] Nara Inst Technol, Grad Sch Informat Sci, Nara, Japan

来源：

JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY | 2013年 / 23卷 / 03期

关键词：

Escherichia coli; sigma(E); Cell lysis; sRNA; Outer membrane protein; Long-term stationary phase; AMINO-ACID CATABOLISM; SMALL NONCODING RNAS; SIGMA(E) REGULON; OUTER-MEMBRANE; GASP PHENOTYPE; RPOE GENE; EXPRESSION; EVOLUTION; BACTERIA; PROTEINS;

D O I：

10.1159/000350370

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

sigma(E)-dependent cell lysis has been proposed to eliminate damaged cells in the stationary phase in Escherichia coli. In order to explore the relationship of this process to long-term stationary phase existence, we considered that micA and rybB could be important small regulatory RNA (sRNA) genes for sigma(E)-dependent cell lysis. A long-term stationary phase was observed at temperatures of <37 degrees C, but not >38 degrees C, and was found even in an rpoS knock-out background. Strains with disrupted micA or rybB were incapable of long-term stationary phase existence. Both strains drastically lost survivability accompanied by a dramatic accumulation of mutations. These findings allow us to speculate that sigma(E)-dependent cell lysis plays a key role in the establishment of the long-term stationary phase, presumably by eliminating damaged cells and thus preventing the over-accumulation of mutations. Copyright (C) 2013 S. Karger AG, Basel

引用

页码：227 / 232

页数：6

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