Phenotypic and molecular characterization of a novel strongly hemolytic Brachyspira species, provisionally designated "Brachyspira hampsonii"

被引:71
作者
Chander, Yogesh [2 ]
Primus, Alexander [1 ]
Oliveira, Simone [1 ]
Gebhart, Connie J. [1 ,3 ]
机构
[1] Univ Minnesota, Minnesota Vet Diagnost Lab, St Paul, MN 55108 USA
[2] Univ Minnesota, Dept Vet Populat Med, St Paul, MN 55108 USA
[3] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA
关键词
16S ribosomal RNA; Brachyspira sp; Brachyspira hampsonii; nox gene; phenotype; swine dysentery; TREPONEMA-HYODYSENTERIAE; SELECTIVE MEDIUM; SWINE-DYSENTERY; PIGS; PILOSICOLI; DISEASE; CHICKENS; SPP; NOV;
D O I
10.1177/1040638712456975
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Since 2007, outbreaks of severe bloody diarrhea and hemorrhagic colitis have been reported in the United States and Canada. Though the primary causative agent of swine dysentery is Brachyspira hyodysenteriae, which is strongly hemolytic, the current report describes the isolation of a novel strongly hemolytic Brachyspira sp. This novel Brachyspira sp. was identified from clinical submissions at the Minnesota Veterinary Diagnostic Laboratory, and 40 of such isolates were obtained from 22 clinical submissions representing 5 states. Isolates were confirmed to be different from any known Brachyspira sp. on the basis of phylogenetic analysis of nucleotide sequences of nox and 16S ribosomal RNA (rRNA) genes. Phylogenetic analyses grouped all isolates into 2 clades (clades I and II), and grouping patterns were similar for both nox and 16S rRNA gene sequence analyses. Phenotypically, all isolates were indole and hippurate negative, and enzymatic profiling indicated 2 types of profiles, irrespective of the phylogenetic grouping, differing only in the production of beta-glucosidase. The results suggest that a potentially virulent new species of Brachyspira sp., provisionally named "Brachyspira hampsonii", is circulating among swine herds in the United States.
引用
收藏
页码:903 / 910
页数:8
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