Protein Kinase A-Dependent Substance P Expression by Pituitary Adenylate Cyclase-Activating Polypeptide in Rat Sensory Neuronal Cell Line ND7/23 Cells

被引：11

作者：

Inoue, Atsuko ^{[1
]}

Ohnishi, Masatoshi ^{[1
]}

Fukutomi, Chiharu ^{[1
]}

Kanoh, Miho ^{[2
]}

Miyauchi, Mutsumi ^{[3
]}

Takata, Takashi ^{[3
]}

Tsuchiya, Daiju ^{[4
]}

Nishio, Hiroaki ^{[4
]}

机构：

[1] Fukuyama Univ, Dept Pharmacotherapeut, Fac Pharm & Pharmaceut Sci, Fukuyama, Hiroshima 7290292, Japan

[2] Hiroshima Univ, Grad Sch Biomed Sci, Dept Pharmacol, Minami Ku, Hiroshima 7348553, Japan

[3] Hiroshima Univ, Grad Sch Biomed Sci, Dept Oral & Maxillofacial Pathobiol, Minami Ku, Hiroshima 7348553, Japan

[4] Fukuyama Univ, Dept Mol Pharmacol, Fac Pharm & Pharmaceut Sci, Fukuyama, Hiroshima 7290292, Japan

来源：

JOURNAL OF MOLECULAR NEUROSCIENCE | 2012年 / 48卷 / 03期

关键词：

PACAP; Substance P; ND7/23; cell; Sensory neuron; Differentiation; CEREBELLAR GRANULE NEURONS; ROOT GANGLION NEURONS; GENE-RELATED PEPTIDE; PACAP; CAMP; DIFFERENTIATION; NEUROPEPTIDES; RECEPTORS; SURVIVAL; BINDING;

D O I：

10.1007/s12031-012-9747-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The neurotrophic effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on rat sensory neuronal cell line ND7/23 cells were investigated. PACAP caused a concentration-dependent increase in the number of neurite-bearing cells and the expression of the substance P precursor (PPT) mRNA in 24 h. The effects of PACAP were mimicked by vasoactive intestinal polypeptide with lower potency and dibutyryl-cyclic AMP, and inhibited by inhibitors of protein kinase A, ERK kinase or p38 kinase, KT5720, U0126, or SB203580, respectively. In a PPT promoter luciferase reporter assay, the increase of PPT mRNA was the result of an increase in PPT gene transcriptional activity by PACAP. The increasing effects of PACAP on PPT mRNA were similarly observed in primary cultured rat dorsal root ganglion cells. Thus, PACAP could induce differentiation-like phenomena in sensory neurons in a cAMP-, protein kinase A-, ERK kinase-, and p38 kinase-dependent manner. These results provide evidence of the neurotrophic action of PACAP, which may function to rescue damaged neurons or to switch the neuronal phenotype in injured or inflamed sensory neurons.

引用

页码：541 / 549

页数：9

共 40 条

[21] Inflammatory pain hypersensitivity mediated by phenotypic switch in myelinated primary sensory neurons [J].

Neumann, S ;

Doubell, TP ;

Leslie, T ;

Woolf, CJ .

NATURE, 1996, 384 (6607) :360-364

[22] PACAP promotes sensory neuron differentiation: blockade by neurotrophic factors [J].

Nielsen, KM ;

Chaverra, M ;

Hapner, SJ ;

Nelson, BR ;

Todd, V ;

Zigmond, RE ;

Lefcort, F .

MOLECULAR AND CELLULAR NEUROSCIENCE, 2004, 25 (04) :629-641

[23] The requirement of Ras and Rap1 for the activation of ERKs by cAMP, PACAP, and KCl in cerebellar granule cells [J].

Obara, Yutaro ;

Horgan, Angela M. ;

Stork, Philip J. S. .

JOURNAL OF NEUROCHEMISTRY, 2007, 101 (02) :470-482

[24] EFFECTS OF INDOMETHACIN, TRIAMCINOLONE, AND DEXAMETHASONE ON RECOMBINANT HUMAN INTERLEUKIN-1-INDUCED SUBSTANCE-P AND PROSTAGLANDIN-E2 LEVELS IN RABBIT KNEE JOINTS [J].

OBYRNE, EM ;

BLANCUZZI, V ;

WILSON, D ;

WONG, M ;

PEPPARD, J ;

SIMKE, JP ;

JENG, AY .

AGENTS AND ACTIONS, 1991, 34 (1-2) :46-48

[25] Changes in expression of PACAP in rat sensory neurons in response to sciatic nerve compression [J].

Pettersson, LME ;

Dahlin, LB ;

Danielsen, N .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2004, 20 (07) :1838-1848

[26] A cAMP-dependent, protein kinase A-independent signaling pathway mediating neuritogenesis through Egr1 in PC12 cells [J].

Ravni, Aurelia ;

Vaudry, David ;

Gerdin, Matthew J. ;

Eiden, Maribeth V. ;

Falluel-Morel, Anthony ;

Gonzalez, Bruno J. ;

Vaudry, Hubert ;

Eiden, Lee E. .

MOLECULAR PHARMACOLOGY, 2008, 73 (06) :1688-1708

[27] Agonistic Behavior of PACAP6-38 on Sensory Nerve Terminals and Cytotrophoblast Cells [J].

Reglodi, D. ;

Borzsei, R. ;

Bagoly, T. ;

Boronkai, A. ;

Racz, B. ;

Tamas, A. ;

Kiss, P. ;

Horvath, G. ;

Brubel, R. ;

Nemeth, J. ;

Toth, G. ;

Helyes, Z. .

JOURNAL OF MOLECULAR NEUROSCIENCE, 2008, 36 (1-3) :270-278

[28] Cellular mechanisms of neurogenic inflammation [J].

Richardson, JD ;

Vasko, MR .

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2002, 302 (03) :839-845

[29] Pituitary Adenylate Cyclase-activating Polypeptide (PACAP) and Vasoactive Intestinal Peptide (VIP) Regulate Murine Neural Progenitor Cell Survival, Proliferation, and Differentiation [J].

Scharf, Eugene ;

May, Victor ;

Braas, Karen M. ;

Shutz, Kristin C. ;

Mao-Draayer, Yang .

JOURNAL OF MOLECULAR NEUROSCIENCE, 2008, 36 (1-3) :79-88

[30] Pituitary adenylate cyclase activating polypeptide:: A potential neuroprotective peptide [J].

Somogyvári-Vigh, A ;

Reglodi, D .

CURRENT PHARMACEUTICAL DESIGN, 2004, 10 (23) :2861-2889

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