Protein Kinase A-Dependent Substance P Expression by Pituitary Adenylate Cyclase-Activating Polypeptide in Rat Sensory Neuronal Cell Line ND7/23 Cells

被引：11

作者：

Inoue, Atsuko ^{[1
]}

Ohnishi, Masatoshi ^{[1
]}

Fukutomi, Chiharu ^{[1
]}

Kanoh, Miho ^{[2
]}

Miyauchi, Mutsumi ^{[3
]}

Takata, Takashi ^{[3
]}

Tsuchiya, Daiju ^{[4
]}

Nishio, Hiroaki ^{[4
]}

机构：

[1] Fukuyama Univ, Dept Pharmacotherapeut, Fac Pharm & Pharmaceut Sci, Fukuyama, Hiroshima 7290292, Japan

[2] Hiroshima Univ, Grad Sch Biomed Sci, Dept Pharmacol, Minami Ku, Hiroshima 7348553, Japan

[3] Hiroshima Univ, Grad Sch Biomed Sci, Dept Oral & Maxillofacial Pathobiol, Minami Ku, Hiroshima 7348553, Japan

[4] Fukuyama Univ, Dept Mol Pharmacol, Fac Pharm & Pharmaceut Sci, Fukuyama, Hiroshima 7290292, Japan

来源：

JOURNAL OF MOLECULAR NEUROSCIENCE | 2012年 / 48卷 / 03期

关键词：

PACAP; Substance P; ND7/23; cell; Sensory neuron; Differentiation; CEREBELLAR GRANULE NEURONS; ROOT GANGLION NEURONS; GENE-RELATED PEPTIDE; PACAP; CAMP; DIFFERENTIATION; NEUROPEPTIDES; RECEPTORS; SURVIVAL; BINDING;

D O I：

10.1007/s12031-012-9747-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The neurotrophic effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on rat sensory neuronal cell line ND7/23 cells were investigated. PACAP caused a concentration-dependent increase in the number of neurite-bearing cells and the expression of the substance P precursor (PPT) mRNA in 24 h. The effects of PACAP were mimicked by vasoactive intestinal polypeptide with lower potency and dibutyryl-cyclic AMP, and inhibited by inhibitors of protein kinase A, ERK kinase or p38 kinase, KT5720, U0126, or SB203580, respectively. In a PPT promoter luciferase reporter assay, the increase of PPT mRNA was the result of an increase in PPT gene transcriptional activity by PACAP. The increasing effects of PACAP on PPT mRNA were similarly observed in primary cultured rat dorsal root ganglion cells. Thus, PACAP could induce differentiation-like phenomena in sensory neurons in a cAMP-, protein kinase A-, ERK kinase-, and p38 kinase-dependent manner. These results provide evidence of the neurotrophic action of PACAP, which may function to rescue damaged neurons or to switch the neuronal phenotype in injured or inflamed sensory neurons.

引用

页码：541 / 549

页数：9

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