Endocytic Pathway and Resistance to Cholesterol Depletion of Cholesterol Derived Cationic Lipids for Gene Delivery

被引：17

作者：

Bae, Yun-Ui ^{[1
]}

Kim, Bieong-Kil ^{[3
]}

Park, Jong-Won ^{[2
]}

Seu, Young-Bae ^{[3
]}

Doh, Kyung-Oh ^{[1
]}

机构：

[1] Yeungnam Univ, Coll Med, Dept Physiol, Taegu 705717, South Korea

[2] Yeungnam Univ, Coll Med, Dept Internal Med, Taegu 705717, South Korea

[3] Kyungpook Natl Univ, Sch Life Sci & Biotechnol, Taegu 702701, South Korea

来源：

MOLECULAR PHARMACEUTICS | 2012年 / 9卷 / 12期

基金：

新加坡国家研究基金会;

关键词：

endocytic pathway; gene delivery; cholesterol; nonviral vector; CAVEOLAE-MEDIATED ENDOCYTOSIS; TRANSFECTION EFFICIENCY; PROTEIN CORONA; INTRACELLULAR TRAFFICKING; DEPENDENT INTERNALIZATION; CELLULAR UPTAKE; OVARIAN-CANCER; IN-VITRO; LIPOPLEXES; CELLS;

D O I：

10.1021/mp300458h

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Cholesterol-based cationic lipids have been widely used because of biocompatibility and serum resistance. However, the reason for the effectiveness of cholesterol-based cationic lipids remains unclear. We compared the transfection route of CHOL-E, a cholesterol-based cationic lipid having an amine head and an ether linker, with that of DOTAP. The luciferase assay with chemical inhibitors and microscopic observation of pathway markers revealed that clathrin mediated endocytosis is the main pathway for CHOL-E and DOTAP. However, CHOL-E showed resistance to cholesterol depletion by methyl-beta-cyclodextrin. Furthermore, CHOL-E recovered the transfection efficiency of DOTAP from cholesterol depletion. These results suggested that superior transfection of CHOL-E might be partly derived from effects on the cell membrane.

引用

页码：3579 / 3585

页数：7

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