The endocannabinoid system mediates aerobic exercise-induced antinociception in rats

被引:66
作者
Galdino, Giovane [1 ]
Romero, Thiago R. L. [1 ]
Silva, Jose Felipe P. [2 ]
Aguiar, Daniele C. [1 ]
de Paula, Ana Maria [3 ]
Cruz, Jader S. [4 ]
Parrella, Cosimo [5 ]
Piscitelli, Fabiana [5 ]
Duarte, Igor D. [1 ]
Di Marzo, Vincenzo [5 ]
Perez, Andrea C. [1 ]
机构
[1] Univ Fed Minas Gerais, Inst Biol Sci, Dept Pharmacol, BR-31270100 Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Inst Biol Sci, Dept Physiol, BR-31270100 Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Dept Phys, Biophoton Lab, BR-31270901 Belo Horizonte, MG, Brazil
[4] Univ Fed Minas Gerais, Inst Biol Sci, Dept Biochem, BR-31270100 Belo Horizonte, MG, Brazil
[5] CNR, Inst Biomol Chem, Endocannabinoid Res Grp, I-80078 Naples, Italy
关键词
Endocannabinoids; Cannabinoid receptors; Aerobic exercise; Pain; Antinociception; FATTY-ACID AMIDE; PERIPHERAL CANNABINOID RECEPTORS; VOLUNTARY EXERCISE; SPINAL-CORD; MOLECULAR CHARACTERIZATION; ANALGESIC ACTIVITY; PHYSICAL-EXERCISE; CB1; PAIN; ACTIVATION;
D O I
10.1016/j.neuropharm.2013.09.022
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Exercise-induced antinociception is widely described in the literature, but the mechanisms involved in this phenomenon are poorly understood. Systemic (s.c.) and central (i.t, i.c.v.) pretreatment with CB1 and CB2 cannabinoid receptor antagonists (AM251 and AM630) blocked the antinociception induced by an aerobic exercise (AE) protocol in both mechanical and thermal nociceptive tests. Western blot analysis revealed an increase and activation of CB1 receptors in the rat brain, and immunofluorescence analysis demonstrated an increase of activation and expression of CB1 receptors in neurons of the periaqueductal gray matter (PAG) after exercise. Additionally, pretreatment (s.c., i.t. and i.c.v.) with endocannabinoid metabolizing enzyme inhibitors (MAFP and JZL184) and an anandamide reuptake inhibitor (VDM11) prolonged and intensified this antinociceptive effect. These results indicate that exercise could activate the endocannabinoid system, producing antinociception. Supporting this hypothesis, liquidchromatography/mass-spectrometry measurements demonstrated that plasma levels of endocannabinoids (anandamide and 2-arachidonoylglycerol) and of anandamide-related mediators (palmitoylethanolamide and oleoylethanolamide) were increased after AE. Therefore, these results suggest that the endocannabinoid system mediates aerobic exercise-induced antinociception at peripheral and central levels. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:313 / 324
页数:12
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