LEGO-NMR Spectroscopy: A Method to Visualize Individual Subunits in Large Heteromeric Complexes

被引：25

作者：

Mund, Markus ^{[1
]}

Overbeck, Jan H. ^{[1
]}

Ullmann, Janina ^{[1
]}

Sprangers, Remco ^{[1
]}

机构：

[1] Max Planck Inst Dev Biol, D-72076 Tubingen, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 43期

关键词：

isotopic labeling; LSm complex; methyl TROSY; NMR spectroscopy; protein structures; SM-LIKE PROTEINS; MOLECULAR-WEIGHT PROTEINS; ESCHERICHIA-COLI; SUPRAMOLECULAR COMPLEXES; RNA-BINDING; CORE DOMAIN; IN-VITRO; U6; SNRNA; YEAST; ARCHITECTURE;

D O I：

10.1002/anie.201304914

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Seeing the big picture: Asymmetric macromolecular complexes that are NMR active in only a subset of their subunits can be prepared, thus decreasing NMR spectral complexity. For the hetero heptameric LSm1-7 and LSm2-8 rings NMR spectra of the individual subunits of the complete complex are obtained, showing a conserved RNA binding site. This LEGO-NMR technique makes large asymmetric complexes accessible to detailed NMR spectroscopic studies. © 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of Creative Commons the Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

引用

收藏

页码：11401 / 11405

页数：5

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