Evaluation of reactive Epstein-Barr Virus (EBV) in Iranian patient with different subtypes of multiple sclerosis (MS)

被引:15
作者
Ramroodi, Nourollah [1 ]
Niazi, Abbas Ali [2 ]
Sanadgol, Nima [3 ,4 ]
Ganjali, Zohre [3 ]
Sarabandi, Vida [3 ]
机构
[1] Zahedan Univ Med Sci, Dept Neurol, Zahedan, Iran
[2] Zahedan Univ Med Sci, Dept Pathol, Zahedan, Iran
[3] Zabol Univ, Fac Sci, Dept Biol, Zabol, Iran
[4] Univ Tehran Med Sci, Cellular & Mol Res Ctr, Tehran, Iran
关键词
EBV; MS; Reactivation; qPCR; ELISA; PATHOGENESIS; INFECTION; RISK; DISEASE; ASSOCIATION; ANTIBODIES; BLOOD; CELLS;
D O I
10.1016/j.bjid.2012.09.008
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: Epstein-Barr virus has been recently associated with the onset of multiple sclerosis, yet understanding how it elicits autoimmunity remains elusive. We investigated the relation between Epstein-Barr virus reactivation and disease development in different subtypes of multiple sclerosis. Methods: In the present research, we have determined the Epstein-Barr virus-DNA load by quantitative real-time polymerase chain reaction and Epstein-Barr virus antibody levels by EIA technique in both multiple sclerosis patients (n = 78) and healthy controls (n = 123). Results: Our results demonstrated increased titer of both anti-Epstein-Barr virus-IgG and IgM antibodies in patients (91.02% vs 82.11% in controls, p < 0.001 and 14.1% vs 4.06% in controls, p < 0.001, respectively). Overall, Epstein-Barr virus reactivation was found in 68.75% of subtypes of multiple sclerosis, 4.54% of multiple sclerosis primary subtype, and in only 3.25% of healthy control subjects. Moreover, in samples of patients with disease relapse (exacerbation) cell free viral DNA was elevated in contrast to other patients (p < 0.001). Conclusions: These findings provide further support for the detrimental effects of Epstein-Barr virus in the reactivation of multiple sclerosis attacks. (C) 2013 Elsevier Editora Ltda. All rights reserved.
引用
收藏
页码:156 / 163
页数:8
相关论文
共 45 条
[1]   Epstein-Barr Virus Infection and Multiple Sclerosis: A Review [J].
Ascherio, Alberto ;
Munger, Kassandra L. .
JOURNAL OF NEUROIMMUNE PHARMACOLOGY, 2010, 5 (03) :271-277
[2]   Identification of human herpesviruses 1 to 8 in Tunisian multiple sclerosis patients and healthy blood donors [J].
Ben Fredj, Nadia ;
Rotola, Antonella ;
Nefzi, Faten ;
Chebel, Saber ;
Rizzo, Roberta ;
Caselli, Elisabetta ;
Frih-Ayed, Mahbouba ;
Di Luca, Dario ;
Aouni, Mahjoub .
JOURNAL OF NEUROVIROLOGY, 2012, 18 (01) :12-19
[3]   Multiple Sclerosis and Viruses [J].
Brahic, Michel .
ANNALS OF NEUROLOGY, 2010, 68 (01) :6-8
[4]   Epstein-Barr virus infection of human brain microvessel endothelial cells: A novel role in multiple sclerosis [J].
Casiraghi, Costanza ;
Dorovini-Zis, Katerina ;
Horwitz, Marc S. .
JOURNAL OF NEUROIMMUNOLOGY, 2011, 230 (1-2) :173-177
[5]   Epstein-Barr virus-specific antibody response in cerebrospinal fluid and serum of patients with multiple sclerosis [J].
Castellazzi, Massimiliano ;
Tamborino, Carmine ;
Cani, Alice ;
Negri, Elena ;
Baldi, Eleonora ;
Seraceni, Silva ;
Tola, Maria Rosaria ;
Granieri, Enrico ;
Contini, Carlo ;
Fainardi, Enrico .
MULTIPLE SCLEROSIS JOURNAL, 2010, 16 (07) :883-887
[6]   Epstein-Barr virus latent membrane protein 2A exacerbates experimental autoimmune encephalomyelitis and enhances antigen presentation function [J].
Chang, Rhoda A. ;
Miller, Stephen D. ;
Longnecker, Richard .
SCIENTIFIC REPORTS, 2012, 2
[7]   Epstein-Barr virus infection. [J].
Cohen, JI .
NEW ENGLAND JOURNAL OF MEDICINE, 2000, 343 (07) :481-492
[8]   Multiple Sclerosis: Risk Factors and their Interactions [J].
Disanto, Giulio ;
Morahan, Julia M. ;
Ramagopalan, Sreeram V. .
CNS & NEUROLOGICAL DISORDERS-DRUG TARGETS, 2012, 11 (05) :545-555
[9]   Autoimmune disease: A role for new anti-viral therapies? [J].
Dreyfus, David H. .
AUTOIMMUNITY REVIEWS, 2011, 11 (02) :88-97
[10]   Controversial Role of Epstein-Barr Virus in Multiple Sclerosis [J].
Fatima, Nazneen ;
Toscano, Michael P. ;
Hunter, Stephen B. ;
Cohen, Cynthia .
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, 2011, 19 (03) :246-252