Intravenous Injections of Human Mesenchymal Stromal Cells Modulated the Redox State in a Rat Model of Radiation Myelopathy

被引：2
作者：
Zhang, Jing [1 ]
Li, Lian-Bing [2 ]
Qiu, Zhu [3 ]
Ren, Hong-Bo [4 ]
Wu, Jia-Yan [5 ]
Wang, Tao [6 ]
Bao, Zhong-Hui [4 ]
Yang, Ji-Fan [4 ]
Zheng, Ke [3 ]
Li, Shao-Lin [7 ]
Wei, Li [2 ]
You, Hua [8 ]
机构：
[1] Liaoning Med Univ, Affiliated Hosp 1, Jinzhou 121000, Liaoning, Peoples R China
[2] Natl Hlth & Family Planning Commiss, Key Lab Birth Defects & Reprod Hlth, Chongqing Populat & Family Planning Sci & Technol, Chongqing 400020, Peoples R China
[3] Chongqing Med Univ, Affiliated Hosp 1, Mol Oncol & Epigenet Lab, Chongqing 400016, Peoples R China
[4] Second Peoples Hosp Banan, Dept Oncol, Chongqing 400054, Peoples R China
[5] Third Mil Med Univ, Xinqiao Hosp, Dept Obstet & Gynecol, Chongqing 400038, Peoples R China
[6] XiAn Gao Xin Hosp, Dept Orthoped 2, Xian 710075, Peoples R China
[7] Chongqing Med Univ, Coll Basic Med Sci, Dept Radiat Med, Chongqing 400016, Peoples R China
[8] Affiliated Hosp, Acad Mil Med Sci, Beijing 100071, Peoples R China
来源：
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2015年 / 2015卷
基金：
中国国家自然科学基金;
关键词：
ENDOTHELIAL GROWTH-FACTOR; SPINAL-CORD CONTUSION; STEM-CELLS; ANGIOPOIETIN-2; IRRADIATION; INJURY; BRAIN;
D O I：
10.1155/2015/432369
中图分类号：
R [医药、卫生];
学科分类号：
10 ;
摘要：
The main aim of the present study was to assess the antioxidative effects of human umbilical cord-derived mesenchymal stromal cells (UC-MSCs) in a rat model of radiation myelopathy. UC-MSCs were isolated from Wharton's jelly (WJ) of umbilical cords. An irradiated cervical spinal cord rat model (C2-T2 segment) was generated using a Co-60 irradiator to deliver 30 Gy of radiation. UC-MSCs were injected through the tail vein at 90 days, 97 days, 104 days, and 111 days after-irradiation. Histological damage was examined by cresyl violet/Nissl staining. The activities of two antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPX) in the spinal cord were measured by the biomedical assay. In addition, the levels of vascular endothelial growth factor (VEGF) and angiopoietin-2 (Ang-2) in the spinal cord were determined by ELISA methods. Multiple injections of UC-MSCs through the tail vein ameliorated neuronal damage in the spinal cord, increased the activities of the antioxidant enzymes CAT and GPX, and increased the levels of VEGF and Ang-2 in the spinal cord. Our results suggest that multiple injections of UC-MSCs via the tail vein in the rat model of radiation myelopathy could significantly improve the antioxidative microenvironment in vivo.
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页数：6
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