An ultrasensitive homogeneous chemiluminescent assay for microRNAs

被引：38

作者：

Deng, Huimin ^{[1
]}

Ren, Yuqian ^{[1
]}

Shen, Wei ^{[1
]}

Gao, Zhiqiang ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 82期

关键词：

CATALYTIC BEACON SENSOR; DNA; SENSITIVITY; IONS; LUMINOL; ENZYME;

D O I：

10.1039/c3cc44824j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

On the basis of a dual-amplification strategy, microRNA (miRNA) hybridization amplification by a duplex-specific nuclease (DSN) and signal amplification by a DNAzyme, a highly sensitive and selective homogeneous miRNA assay is developed. By leveraging on the cumulative and selective nature of the DSN amplification process, excellent sensitivity and selectivity are accomplished.

引用

页码：9401 / 9403

页数：3

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