RAPD analysis of somaclonal variants derived from embryo callus cultures of peach

被引：0
作者：
Hashmi, G
Huettel, R
Meyer, R
Krusberg, L
Hammerschlag, F
机构：
[1] USDA ARS, BELTSVILLE AGR RES CTR, FRUIT LAB, BELTSVILLE, MD 20705 USA
[2] USDA ARS, BELTSVILLE AGR RES CTR, PLANT MOL BIOL LAB, BELTSVILLE, MD 20705 USA
[3] USDA ARS, BELTSVILLE AGR RES CTR, NEMATOL LAB, BELTSVILLE, MD 20705 USA
[4] UNIV MARYLAND, DEPT BOT, COLLEGE PK, MD 20742 USA
来源：
PLANT CELL REPORTS | 1997年 / 16卷 / 09期
关键词：
Prunus persica; somaclonal variation;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Peach [Prunus persica (L.) Batsch] regenerants from cv 'Sunhigh' embryo no. 156, regenerants obtained from cv 'Redhaven' embryo no. 30, and two peach cultivars 'Sunhigh' and 'Redhaven', were screened for polymorphic RAPD (Random Amplified Polymorphic DNA) markers with up to 60 IO-mer primers. Although 35 primers produced results with scoreable bands, only 10 of the primers revealed polymorphism for regenerants of embryo no.156 and cv 'Sunhigh', and 1 revealed a low level of polymorphism for regenerants of embryo no. 30 and cv 'Redhaven'. This study demonstrates the feasibility of using RAPD markers to identify somaclonal variants of peach and provides evidence for the existence of genetic differences among these variants.
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收藏
页码：624 / 627
页数：4
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