Antinociceptive effects of oxymatrine from Sophora flavescens, through regulation of NR2B-containing NMDA receptor-ERK/CREB signaling in a mice model of neuropathic pain

被引：29
作者：
Wang Haiyan [1 ,4 ]
Li Yuxiang [2 ,5 ]
Dun Linglu [1 ]
Xu Yaqiong [1 ]
Jin Shaojv [1 ]
Du Juan [1 ]
Ma Lin [3 ]
Li Juan [1 ]
Zhou Ru [1 ]
He Xiaoliang [1 ]
Sun Tao [3 ]
Yu Jianqiang [1 ]
机构：
[1] Ningxia Med Univ, Dept Pharmacol, Yinchuan, Peoples R China
[2] Ningxia Med Univ, Coll Nursing, Yinchuan, Peoples R China
[3] Ningxia Key Lab Craniocerbral Dis Ningxia Hui Aut, Yinchuan, Peoples R China
[4] Shanghai Jiao Tong Univ, Sch Med, Chongming Branch, Xin Hua Hosp, Shanghai 200030, Peoples R China
[5] Shanghai Pudong New Area Gongli Hosp, Shanghai, Peoples R China
来源：
PHYTOMEDICINE | 2013年 / 20卷 / 11期
关键词：
Oxymatrine from Sophora flavescens; Chronic constrictive injury; NR2B; p-ERK; p-CREB; Neuropathic pain; CHRONIC CONSTRICTION INJURY; SCIATIC-NERVE LIGATION; BINDING PROTEIN CREB; DORSAL-HORN NEURONS; GLUTAMATE RECEPTORS; TACTILE ALLODYNIA; SPINAL-CORD; PHOSPHORYLATION; ACTIVATION; EXPRESSION;
D O I：
10.1016/j.phymed.2013.04.012
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Purpose: In this study we investigated antinociceptive effects of oxymatrine through regulation of NR2B-containing NMDA receptor-ERK/CREB signaling in a chronic neuropathic pain model induced by chronic constrictive injury (CCI) of the sciatic nerve. Methods: The von Frey and plantar tests were performed to assess the degree of mechanical and thermal changes respectively. Immunohistochemistry assay was used to evaluate the expressions of NR2B. Western blotting assay were used to evaluate the expressions of NR2B, tERK, p-ERK, tCREB and p-CREB. Results: The intraperitoneal administration of OMT (160, 80 mg/kg) could prevent the development of mechanical allodynia, thermal hyperalgesia induced by CCI. Intraperitoneal administration of OMT decreased the mean IOD of NR2B in the dorsal horn and expression of NR2B, p-ERK and p-CREB protein. Conclusion: Regulation of NMDA NR2B receptor-ERK/CREB signaling maybe the targets for the antinociceptive effects of OMT on a chronic neuropathic pain model induced by chronic constrictive injury of the sciatic nerve. (C) 2013 Elsevier GmbH. All rights reserved.
引用
收藏
页码：1039 / 1045
页数：7
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