Characterization of phenol-degrading bacterial strains isolated from natural soil

被引：67

作者：

Dong, Xiaojun ^{[1
]}

Hong, Qing ^{[1
,2
]}

He, Lijuan ^{[1
]}

Jiang, Xin ^{[2
]}

Li, Shunpeng ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Life Sci, Key Lab Microbiol Engn Agr Environm, Minist Agr, Nanjing 210095, Peoples R China

[2] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2008年 / 62卷 / 03期

基金：

中国博士后科学基金;

关键词：

Phenol; Degradation; LmPH; Molecular marker; Metabolism pathway;

D O I：

10.1016/j.ibiod.2008.01.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Six phenol-degrading bacteria designated as PND-1-PND-6 were isolated from natural soil. PND-1-PND-6 were from the genera of Pseudomonas, Acinetobacter, Comamonas and Cupriavidus, respectively. All these strains were able to utilize phenol as the sole carbon source to support their growth. Five of them could tolerate the phenol concentration up to 6 mM or more, while strain PND-3 could tolerate only 1 mM of phenol. The sequences of the partial largest subunit of multicomponent phenol hydroxylase (LmPH) gene were compared among these strains. It was found that the physiological groupings in the phylogenetic tree formed by their 16S rDNA sequences were correlated with that of based on their partial amino acid sequences of LmPH, which indicated the potential application of LmPH as a molecular marker for the phylogenetic analysis of phenol-degrading strains. Four of the six strains degraded phenol through catechol ortho fission pathway, whereas strain PND-3 and PND-6 harbored the both ortho and meta fission pathways simultaneously. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：257 / 262

页数：6

共 31 条

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[2]

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