Identification of the actual coding region for polyamine oxidase 6 from rice (OsPAO6) and its partial characterization

被引：11
|
作者：
Sagor, G. H. M. [1 ,2 ,3 ]
Kusano, Tomonobu [2 ]
Berberich, Thomas [1 ]
机构：
[1] Senckenberg Biodivers & Climate Res Ctr, Lab Ctr, Georg Voigt Str 4-16, D-60320 Frankfurt, Hesse, Germany
[2] Tohoku Univ, Grad Sch Life Sci, Sendai, Miyagi, Japan
[3] Bangladesh Agr Univ, Fac Agr, Dept Genet & Plant Breeding, Mymensingh, Bangladesh
来源：
PLANT SIGNALING & BEHAVIOR | 2017年 / 12卷 / 08期
基金：
日本学术振兴会;
关键词：
Polyamines; polyamine oxidase; rice; Oryza sativa; coding region; cDNA; catabolism;
D O I：
10.1080/15592324.2017.1359456
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Polyamines (PA) in plant play roles in growth and development and in responses to environmental stresses. The family of polyamine oxidases (PAO) contributes to a balanced homeostasis of PAs catalyzing two different reactions, terminal catabolic (TC) and back-conversion (BC) pathway, in PA catabolism. From the seven PAOs encoded by the rice genome (OsPAO1 - OsPAO7) OsPAO6 could so far not be characterized due to failure in obtaining the coding cDNA based on accessions in the genomic databases. We report cloning and characterization of the correct OsPAO6 cDNA with a length of 1,742 bp. The 1,491 bp long open reading frame codes for a 497-amino acid protein from nine exons. The protein which has 92% identity to OsPAO7 localizes to plasma membrane.
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页数：3
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