A Rapid and Efficient Agrobacterium-Mediated Transformation Protocol for Lilium

被引：0

作者：

de Caceres, F. F. Nunez ^{[1
]}

Davey, M. R. ^{[1
]}

Wilson, Z. A. ^{[1
]}

机构：

[1] Univ Nottingham, Plant & Crop Sci Div, Loughborough LE12 5RD, Leics, England

来源：

II INTERNATIONAL SYMPOSIUM ON THE GENUS LILIUM | 2011年 / 900卷

关键词：

Agrobacterium tumefaciens; beta-glucuronidase (GUS); neomycin phosphotransferase II (nptII); Lilium 'Star Gazer'; PARTICLE BOMBARDMENT; LONGIFLORUM; EXPRESSION; POLLEN; PLANTS; GENE;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A protocol has been developed for the clonal propagation and Agrobacterium-mediated transformation of the Lilium oriental hybrid 'Star Gazer'. Transient GUS expression in basal plate disc-derived tissues and regenerated shoots was achieved following transformation using A. tumefaciens strain AGL1 containing pBI121, the latter carrying the beta-glucuronidase (gus) and neomycin phosphotransferase II (nptII) genes. A mean transformation efficiency of 20% was achieved in three independent transformation experiments. Stable transgene integration and expression were confirmed by PCR and RT-PCR analyses. The efficiency, reproducibility and rapidity of this protocol make it suitable for commercial application and for future research on this important ornamental plant.

引用

页码：161 / 167

页数：7

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