Phosphorylated AKT Protein Is Overexpressed in Human Peripheral T-cell Lymphomas and Predicts Decreased Patient Survival

被引：17
作者：
Cai, Qingqing [2 ]
Deng, Haixia [2 ]
Xie, Dan [2 ]
Lin, Tianxin [3 ,4 ]
Lin, Tongyu [1 ,2 ]
机构：
[1] Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol So China, Dept Med Oncol, Guangzhou 510060, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Affiliated Hosp 2, State Key Lab Oncol So China, Guangzhou 510060, Guangdong, Peoples R China
[3] Sun Yat Sen Univ, Affiliated Hosp 2, Dept Urol, Guangzhou 510060, Guangdong, Peoples R China
[4] Sun Yat Sen Univ, Affiliated Hosp 2, Lin Bai Xin Med Res Ctr, Guangzhou 510060, Guangdong, Peoples R China
来源：
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA | 2012年 / 12卷 / 02期
关键词：
Immunohistochemistry; Peripheral T-cell lymphoma; Phosphorylated AKT; Prognosis; LUNG-CANCER CELLS; NF-KAPPA-B; CHEMOTHERAPEUTIC RESISTANCE; MULTIDRUG-RESISTANCE; PI3K/AKT PATHWAY; PROSTATE-CANCER; KINASE-B; ACTIVATION; EXPRESSION; PTEN;
D O I：
10.1016/j.clml.2011.12.002
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The expression of phosphorylated AKT (pAKT) in peripheral T-cell lymphomas (PTCL) and its clinical significance were studied. We detected pAKT expression by using the immunohistochemical method in 106 cases of PTCL. Positive pAKT expression was detected in 49.1% of patients, was negatively correlated with overall response rate, and predicted decreased progression-free survival and/or overall survival. Our results suggest that pAKT expression is an independent prognostic factor for PTCL. Background: The AKT signaling pathway controls the survival and growth of human cancer cells. The purpose of this study was to investigate the expression dynamics of phosphorylated AKT (pAKT) in peripheral T-cell lymphoma (PTCL) and its clinical significance. Materials and Methods: Immunohistochemistry was performed to examine the pAKT expression in 106 PTCL cases. pAKT expression and its association with clinicopathologic characteristics of a patient with PTCL were analyzed. Results: A total of 106 samples were tested, and 52 (49.1%) were positive for pAKT. There was significant positive correlation between pAKT expression and higher levels of lactic dehydrogenase (P<.05), whereas positive pAKT expression was negatively correlated with patients' overall response rate (P<.05). Further multivariate analysis revealed that positive pAKT expression predicted decreased progression-free survival and/or overall survival (P<.05). Conclusion: Analysis of our results suggest that pAKT expression, as examined by immunohistochemistry, is an independent prognostic factor for PTCL. The clinical value of pAKT expression analysis in PTCL is worthy of further clinical investigations.
引用
收藏
页码：106 / 112
页数：7
相关论文
共 35 条
[1]   Mechanism of activation and function of protein kinase B [J].
Alessi, DR ;
Cohen, P .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 1998, 8 (01) :55-62
[2]   Perturbations of the AKT signaling pathway in human cancer [J].
Altomare, DA ;
Testa, JR .
ONCOGENE, 2005, 24 (50) :7455-7464
[3]   PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2 [J].
Balendran, A ;
Casamayor, A ;
Deak, M ;
Paterson, A ;
Gaffney, P ;
Currie, R ;
Downes, CP ;
Alessi, DR .
CURRENT BIOLOGY, 1999, 9 (08) :393-404
[4]   LY294,002, a specific inhibitor of PI3K/Akt kinase pathway, antagonizes P-glycoprotein-mediated multidrug resistance [J].
Barancik, Miroslav ;
Bohacova, Vierka ;
Sedlak, Jan ;
Sulova, Zdenka ;
Breier, Albert .
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2006, 29 (05) :426-434
[5]   Determining risk of biochemical recurrence in prostate cancer by immunohistochemical detection of PTEN expression end Akt activation [J].
Bedolla, Roble ;
Prihoda, Thomas J. ;
Kreisberg, Jeffrey I. ;
Malik, Shazli N. ;
Krishnegowda, Naveen K. ;
Troyer, Dean A. ;
Ghosh, Paramita M. .
CLINICAL CANCER RESEARCH, 2007, 13 (13) :3860-3867
[6]   Activation of AKT kinases in cancer: Implications for therapeutic targeting [J].
Bellacosa, A ;
Kumar, CC ;
Di Cristofano, A ;
Testa, JR .
ADVANCES IN CANCER RESEARCH, VOL 94, 2005, 94 :29-+
[7]   Variable apoptotic response of NSCLC cells to inhibition of the MEK/ERK pathway by small molecules or dominant negative mutants [J].
Brognard, J ;
Dennis, PA .
CELL DEATH AND DIFFERENTIATION, 2002, 9 (09) :893-904
[8]  
Brognard J, 2001, CANCER RES, V61, P3986
[9]   Akt promotes cell survival by phosphorylating and inhibiting a forkhead transcription factor [J].
Brunet, A ;
Bonni, A ;
Zigmond, MJ ;
Lin, MZ ;
Juo, P ;
Hu, LS ;
Anderson, MJ ;
Arden, KC ;
Blenis, J ;
Greenberg, ME .
CELL, 1999, 96 (06) :857-868
[10]   Regulation of cell death protease caspase-9 by phosphorylation [J].
Cardone, MH ;
Roy, N ;
Stennicke, HR ;
Salvesen, GS ;
Franke, TF ;
Stanbridge, E ;
Frisch, S ;
Reed, JC .
SCIENCE, 1998, 282 (5392) :1318-1321
1234